β-环糊精共轭丁蛋白作为胆碱的选择性电化学分子运输系统

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X Pub Date : 2025-06-18 DOI:10.1016/j.biosx.2025.100647

Ramya Kanagaraj , Shanmugam SenthilKumar , Gururaja Perumal Pazhani , Murugan Veerapandian

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引用次数: 0

摘要

监测抗组胺药物bilastine （BIL）治疗有效浓度的简单方法，对变态反应和呼吸治疗的合理化至关重要。本研究利用偶联低聚糖-多酚体系β-环糊精-丁蛋白（BCD-Bt）建立了一种无氧化还原介质的外部分子运输方法。利用循环和差分脉冲伏安分析探讨了BCD疏水腔内BIL分子负载的机制，同时使共轭Bt的氧化还原活性信号转导成为可能。BCD-Bt传感器平台可选择性负载分析物BIL，具有可靠的检测线性（nm ~ μM, R2 = 0.981）和可行的唾液治疗药物监测限。协同光学吸收和基于发射的分子运输测试进一步补充了BCD-Bt在其他类似分子分析中的多模态功能。

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β-Cyclodextrin-conjugated butein as a selective electrochemical molecular trafficking system for bilastine

Simple method for monitoring the therapeutic efficient concentration of anti-histamine drug, bilastine (BIL), is essential for rationalizing allergy and respiratory therapy. Herein, an external redox-mediator free molecular trafficking approach was developed using conjugated oligosaccharide-polyphenol system, β-cyclodextrin-butein (BCD-Bt). Mechanism behind the molecular loading of BIL within the hydrophobic cavity of BCD simultaneously enabling the redox-active signal transduction from the conjugated Bt are explored using cyclic and differential pulse voltammetric analyses. BCD-Bt sensor platform exemplified the selective loading of analyte BIL with a reliable detection linearity (nm to μM, R² = 0.981) and limit feasible for salivary therapeutic drug monitoring. Synergistic optical absorbance and emission-based molecular trafficking test further complements the multi-modal functionality of the demonstrated BCD-Bt in other similar molecular assays.

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来源期刊

Biosensors and Bioelectronics: X Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

166

审稿时长

54 days

期刊介绍： Biosensors and Bioelectronics: X, an open-access companion journal of Biosensors and Bioelectronics, boasts a 2020 Impact Factor of 10.61 (Journal Citation Reports, Clarivate Analytics 2021). Offering authors the opportunity to share their innovative work freely and globally, Biosensors and Bioelectronics: X aims to be a timely and permanent source of information. The journal publishes original research papers, review articles, communications, editorial highlights, perspectives, opinions, and commentaries at the intersection of technological advancements and high-impact applications. Manuscripts submitted to Biosensors and Bioelectronics: X are assessed based on originality and innovation in technology development or applications, aligning with the journal's goal to cater to a broad audience interested in this dynamic field.