Antioxidant property of Silver Nanoparticles Loaded with Alcoholic Extraction of Lycium Shawii.

Background: Antioxidants play a crucial role in mitigating oxidative stress associated with cancer progression. Lycium shawii is noted for its high content of antioxidants and anti-inflammatory compounds. Silver nanoparticles (AgNPs) hold a prominent position among the various nanoparticles synthesized and characterized to date.

Objective: In this study, we aimed to synthesize and characterize AgNPs loaded with alcoholic extracts of Lycium shawii and evaluate their antioxidant effects in vivo.

Methods: Thirty rats were randomly divided into five groups, with six rats in each group. Group 1 (Control) was administered 1 mL of normal saline orally via a stomach tube daily. Group 2 received 1 mL of AgNPs at a dosage of 100 mg/kg on the day of administration. Group 3 was treated with 1 mL/day of Acetamiprid at a dosage of 25 mg/kg. Group 4 was given AgNPs (100 mg/kg) for three weeks, followed by Acetamiprid (25 mg/kg) for an additional three weeks. Group 5 received Acetamiprid (25 mg/kg) for three weeks, followed by AgNPs (100 mg/kg). All treatments were administered daily, and serum levels of T-AOC, TOS, as well as the biomarkers AST, ALT, urea, and creatinine, were evaluated across all groups.

Results: The results of the study demonstrated a significant increase in TOS, ALT, AST, urea, and creatinine, as well as a significant decrease in T-AOC (p ≤ 0.05) in Group 3 compared to Groups 1, 2, 4, and 5.

Conclusion: The in vivo results demonstrate that AgNPs, especially when combined with Lycium shawii extracts, exhibit significant antioxidant properties, suggesting a protective role against oxidative damage. These findings support the therapeutic potential of AgNPs in cancer treatment and oxidative stress-related disorders. Future studies are needed to fully elucidate the molecular mechanisms underlying the observed antioxidant effects and to assess the long-term safety and efficacy of AgNPs in clinical applications.