半夏块茎中的生物碱。

IF 3.4 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Phytochemistry Pub Date : 2025-06-19 DOI:10.1016/j.phytochem.2025.114597

Xu Chen , Yan-gang Cao , Feng-xiao Hao , Yan-ling Liu , Fang-ge Chi , Ying Niu , Di Lu , Bing-xian Zhao , Lan Chen , Xiao-ke Zheng , Wei-sheng Feng

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引用次数: 0

摘要

从半夏（Pinellia pedatisecta Schott）块茎中分离得到9个未描述的生物碱（1、3、4、7、8和10-13）和4个未描述的天然产物（2、5、6和9），以及8个已知成分。通过光谱数据、Rh2(OCOCF3)4诱导ECD光谱分析、DP4+分析、ECD分析以及旋光度数据的解释对其结构进行了鉴定。采用MTT法检测各化合物对a β25-35诱导的PC-12细胞损伤的保护作用，结果表明化合物1、2、5-10和16-20具有明显的保护作用。此外，通过流式细胞术和细胞免疫荧光研究了化合物2和5的作用机制，结果表明化合物2和5通过NMDAR/p-CamK II/PKG信号通路改善了a - β25-35诱导的PC-12细胞损伤。本研究为今后研究pedatisecta的化学成分及开发治疗阿尔茨海默病的天然药物提供了实验依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Alkaloids from the tubers of Pinellia pedatisecta

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Alkaloids from the tubers of Pinellia pedatisecta

Nine undescribed alkaloids (1, 3, 4, 7, 8 and 10–13) and four undescribed natural products (2, 5, 6 and 9), together with eight known components, were isolated from the tubers of Pinellia pedatisecta Schott. Their structures were elucidated based on spectroscopic data, Rh₂(OCOCF₃)₄-induced ECD spectral analysis, DP4+ analysis, ECD analysis and interpretation of their optical rotation data. The protective effects of all compounds against Aβ_25-35-induced PC-12 cell injury were tested by MTT method, and the results indicated that compounds 1, 2, 5–10 and 16–20 exhibited obviously protective effects. In addition, the action mechanisms of compounds 2 and 5 were investigated by flow cytometry and cellular immunofluorescence, and the results indicated that compounds 2 and 5 ameliorated Aβ_25-35-induced PC-12 cell injury via the NMDAR/p-CamK II/PKG signaling pathway. This study provided experimental basis for researching the chemical composition of P. pedatisecta and developing natural medicines for the treatment of Alzheimer's disease in the future.

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来源期刊

Phytochemistry 生物-植物科学

CiteScore

6.40

自引率

7.90%

发文量

443

审稿时长

39 days

期刊介绍： Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.