Chen Yuanyuan, Sun Ningyu, Lu Lu, Zhang Wuwen, Li Kai, Li Yanting, He Junlan, Cheng Xiang, Zhou Jing, Yan Hua, Yin Ping
{"title":"多囊卵巢综合征大鼠模型中卵泡膜细胞的转录组学分析：对卵泡发育的见解。","authors":"Chen Yuanyuan, Sun Ningyu, Lu Lu, Zhang Wuwen, Li Kai, Li Yanting, He Junlan, Cheng Xiang, Zhou Jing, Yan Hua, Yin Ping","doi":"10.1186/s13048-025-01663-w","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>This study aimed to analyze differentially expressed genes in theca cells of polycystic ovary syndrome (PCOS) rats using transcriptomic sequencing. Bioinformatics analysis and PCR validation were performed to identify genes involved in follicular development regulation in PCOS.</p><p><strong>Methods: </strong>Twenty 6-week-old female SD rats with regular estrous cycles were divided into two groups (PCOS and control, n = 10 each). The PCOS model was induced with a 1.0 mg·kg⁻¹ Letrozole solution. Theca cells were collected for transcriptomic sequencing, and differentially expressed genes were analyzed. Functional annotations and pathway enrichment were determined using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Gene interaction network and hub gene analyses were conducted, followed by RT-qPCR validation.</p><p><strong>Results: </strong>PCOS rats exhibited increased body weight and irregular estrous cycles. A total of 1,114 differentially expressed genes were identified, including 516 upregulated and 598 downregulated genes. Fifty hub genes were selected for further analysis. GO and KEGG pathway enrichment analysis revealed significant involvement of the MAPK and PI3K-Akt signaling pathways. PCR validation confirmed that Cyp17a1, Cyp11a1, S6k1, mTOR, Akt, Kit, and Tek were significantly upregulated in the PCOS group (P < 0.05).</p><p><strong>Conclusion: </strong>Transcriptomic analysis identified key genes and pathways involved in follicular development dysregulation in PCOS rats, providing potential targets for further research.</p>","PeriodicalId":16610,"journal":{"name":"Journal of Ovarian Research","volume":"18 1","pages":"135"},"PeriodicalIF":3.8000,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transcriptomic analysis of theca cells in a PCOS rat model: insights into follicular development.\",\"authors\":\"Chen Yuanyuan, Sun Ningyu, Lu Lu, Zhang Wuwen, Li Kai, Li Yanting, He Junlan, Cheng Xiang, Zhou Jing, Yan Hua, Yin Ping\",\"doi\":\"10.1186/s13048-025-01663-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>This study aimed to analyze differentially expressed genes in theca cells of polycystic ovary syndrome (PCOS) rats using transcriptomic sequencing. Bioinformatics analysis and PCR validation were performed to identify genes involved in follicular development regulation in PCOS.</p><p><strong>Methods: </strong>Twenty 6-week-old female SD rats with regular estrous cycles were divided into two groups (PCOS and control, n = 10 each). The PCOS model was induced with a 1.0 mg·kg⁻¹ Letrozole solution. Theca cells were collected for transcriptomic sequencing, and differentially expressed genes were analyzed. Functional annotations and pathway enrichment were determined using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Gene interaction network and hub gene analyses were conducted, followed by RT-qPCR validation.</p><p><strong>Results: </strong>PCOS rats exhibited increased body weight and irregular estrous cycles. A total of 1,114 differentially expressed genes were identified, including 516 upregulated and 598 downregulated genes. Fifty hub genes were selected for further analysis. GO and KEGG pathway enrichment analysis revealed significant involvement of the MAPK and PI3K-Akt signaling pathways. PCR validation confirmed that Cyp17a1, Cyp11a1, S6k1, mTOR, Akt, Kit, and Tek were significantly upregulated in the PCOS group (P < 0.05).</p><p><strong>Conclusion: </strong>Transcriptomic analysis identified key genes and pathways involved in follicular development dysregulation in PCOS rats, providing potential targets for further research.</p>\",\"PeriodicalId\":16610,\"journal\":{\"name\":\"Journal of Ovarian Research\",\"volume\":\"18 1\",\"pages\":\"135\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-06-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Ovarian Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1186/s13048-025-01663-w\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"REPRODUCTIVE BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Ovarian Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s13048-025-01663-w","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"REPRODUCTIVE BIOLOGY","Score":null,"Total":0}
Transcriptomic analysis of theca cells in a PCOS rat model: insights into follicular development.
Background: This study aimed to analyze differentially expressed genes in theca cells of polycystic ovary syndrome (PCOS) rats using transcriptomic sequencing. Bioinformatics analysis and PCR validation were performed to identify genes involved in follicular development regulation in PCOS.
Methods: Twenty 6-week-old female SD rats with regular estrous cycles were divided into two groups (PCOS and control, n = 10 each). The PCOS model was induced with a 1.0 mg·kg⁻¹ Letrozole solution. Theca cells were collected for transcriptomic sequencing, and differentially expressed genes were analyzed. Functional annotations and pathway enrichment were determined using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Gene interaction network and hub gene analyses were conducted, followed by RT-qPCR validation.
Results: PCOS rats exhibited increased body weight and irregular estrous cycles. A total of 1,114 differentially expressed genes were identified, including 516 upregulated and 598 downregulated genes. Fifty hub genes were selected for further analysis. GO and KEGG pathway enrichment analysis revealed significant involvement of the MAPK and PI3K-Akt signaling pathways. PCR validation confirmed that Cyp17a1, Cyp11a1, S6k1, mTOR, Akt, Kit, and Tek were significantly upregulated in the PCOS group (P < 0.05).
Conclusion: Transcriptomic analysis identified key genes and pathways involved in follicular development dysregulation in PCOS rats, providing potential targets for further research.
期刊介绍：
Journal of Ovarian Research is an open access, peer reviewed, online journal that aims to provide a forum for high-quality basic and clinical research on ovarian function, abnormalities, and cancer. The journal focuses on research that provides new insights into ovarian functions as well as prevention and treatment of diseases afflicting the organ.
Topical areas include, but are not restricted to:
Ovary development, hormone secretion and regulation
Follicle growth and ovulation
Infertility and Polycystic ovarian syndrome
Regulation of pituitary and other biological functions by ovarian hormones
Ovarian cancer, its prevention, diagnosis and treatment
Drug development and screening
Role of stem cells in ovary development and function.
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