Krzysztof M. Bąk, Daniel C. Edwards, Dylan George, Bhanu Singh, Ryan Ferguson, Tianxiao Zhao, Kristin Piché, Ariel Louwrier, Scott L. Cockroft, Mathew H. Horrocks
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引用次数: 0
摘要
Scott L. Cockroft、Mathew H. Horrocks等人在他们的研究文章（e202503678）中报道了使用共聚焦显微镜和快流微流体装置进行膜渗透的单分子脂质体检测。脂质体包裹着钙敏感染料，通过与显微镜对齐的通道。膜渗透剂允许钙流入，使脂质体通过共聚焦体积时检测到的染料增敏。当连续的脂质体通过体积时，获得的信号表明分析物的渗透特性。
Outside Back Cover: A Single-Molecule Liposome Assay for Membrane Permeabilisation (Angew. Chem. 26/2025)
A single-molecule liposome assay for membrane permeabilization using confocal microscopy paired with a fast-flow microfluidics device is reported by Scott L. Cockroft, Mathew H. Horrocks et al. in their Research Article (e202503678). Liposomes, encapsulating calcium-sensitive dye, pass through the channel aligned with the microscope. Membrane-permeabilizing agents allow calcium influx, sensitizing the dye which is detected as the liposome passes through the confocal volume. As successive liposomes pass through the volume, signals acquired indicate the permeabilising properties of the analyte.
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