Huanling Wu, Chang Liu, Siqin Li, Chenchen Zhang, Guang Yang, Jiang Ma, Ziying Huang, Shixiong Wang, Yonghao Xu, Xin He and Ji Yang
{"title":"Isodon serra属地的二萜类化合物及其抗肝癌潜能","authors":"Huanling Wu, Chang Liu, Siqin Li, Chenchen Zhang, Guang Yang, Jiang Ma, Ziying Huang, Shixiong Wang, Yonghao Xu, Xin He and Ji Yang","doi":"10.1039/D5RA02720A","DOIUrl":null,"url":null,"abstract":"<p >Three new <em>ent</em>-kaurane diterpenoids, isodosins E–G (<strong>1–3</strong>), along with 20 known ones (<strong>4–23</strong>), were obtained from the aerial parts of <em>Isodon serra</em> (Maxim.) Hara. The structures of the new compounds were elucidated using 1D/2D NMR spectra and HREIMS data, and their absolute configurations were determined by electronic circular dichroism (ECD) calculations. The <em>in vitro</em> anti-hepatocarcinoma activities of compounds <strong>2</strong>, <strong>3</strong>, <strong>5</strong>, <strong>8</strong>, <strong>13</strong>, <strong>19</strong>, and <strong>23</strong> were evaluated against HepG2 and Huh7 cell lines using the CCK-8 assay. Among them, compounds <strong>3</strong>, <strong>8</strong>, and <strong>23</strong> exhibited high inhibitory effects on HepG2 cells, with IC<small><sub>50</sub></small> values of 6.94 ± 9.10 μM, 71.66 ± 10.81 μM, and 43.26 ± 9.07 μM, respectively. In a Hepa1-6 xenograft mouse model, compound <strong>8</strong> significantly inhibited tumor growth at doses of 50 and 100 mg kg<small><sup>−1</sup></small>, demonstrating its potent anti-hepatocarcinoma activity.</p>","PeriodicalId":102,"journal":{"name":"RSC Advances","volume":" 25","pages":" 20134-20142"},"PeriodicalIF":4.6000,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ra/d5ra02720a?page=search","citationCount":"0","resultStr":"{\"title\":\"Diterpenoids from the aerial parts of Isodon serra and their anti-hepatocarcinoma potential†\",\"authors\":\"Huanling Wu, Chang Liu, Siqin Li, Chenchen Zhang, Guang Yang, Jiang Ma, Ziying Huang, Shixiong Wang, Yonghao Xu, Xin He and Ji Yang\",\"doi\":\"10.1039/D5RA02720A\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Three new <em>ent</em>-kaurane diterpenoids, isodosins E–G (<strong>1–3</strong>), along with 20 known ones (<strong>4–23</strong>), were obtained from the aerial parts of <em>Isodon serra</em> (Maxim.) Hara. The structures of the new compounds were elucidated using 1D/2D NMR spectra and HREIMS data, and their absolute configurations were determined by electronic circular dichroism (ECD) calculations. The <em>in vitro</em> anti-hepatocarcinoma activities of compounds <strong>2</strong>, <strong>3</strong>, <strong>5</strong>, <strong>8</strong>, <strong>13</strong>, <strong>19</strong>, and <strong>23</strong> were evaluated against HepG2 and Huh7 cell lines using the CCK-8 assay. Among them, compounds <strong>3</strong>, <strong>8</strong>, and <strong>23</strong> exhibited high inhibitory effects on HepG2 cells, with IC<small><sub>50</sub></small> values of 6.94 ± 9.10 μM, 71.66 ± 10.81 μM, and 43.26 ± 9.07 μM, respectively. In a Hepa1-6 xenograft mouse model, compound <strong>8</strong> significantly inhibited tumor growth at doses of 50 and 100 mg kg<small><sup>−1</sup></small>, demonstrating its potent anti-hepatocarcinoma activity.</p>\",\"PeriodicalId\":102,\"journal\":{\"name\":\"RSC Advances\",\"volume\":\" 25\",\"pages\":\" 20134-20142\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2025-06-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://pubs.rsc.org/en/content/articlepdf/2025/ra/d5ra02720a?page=search\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"RSC Advances\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2025/ra/d5ra02720a\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"RSC Advances","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/ra/d5ra02720a","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Diterpenoids from the aerial parts of Isodon serra and their anti-hepatocarcinoma potential†
Three new ent-kaurane diterpenoids, isodosins E–G (1–3), along with 20 known ones (4–23), were obtained from the aerial parts of Isodon serra (Maxim.) Hara. The structures of the new compounds were elucidated using 1D/2D NMR spectra and HREIMS data, and their absolute configurations were determined by electronic circular dichroism (ECD) calculations. The in vitro anti-hepatocarcinoma activities of compounds 2, 3, 5, 8, 13, 19, and 23 were evaluated against HepG2 and Huh7 cell lines using the CCK-8 assay. Among them, compounds 3, 8, and 23 exhibited high inhibitory effects on HepG2 cells, with IC50 values of 6.94 ± 9.10 μM, 71.66 ± 10.81 μM, and 43.26 ± 9.07 μM, respectively. In a Hepa1-6 xenograft mouse model, compound 8 significantly inhibited tumor growth at doses of 50 and 100 mg kg−1, demonstrating its potent anti-hepatocarcinoma activity.
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An international, peer-reviewed journal covering all of the chemical sciences, including multidisciplinary and emerging areas. RSC Advances is a gold open access journal allowing researchers free access to research articles, and offering an affordable open access publishing option for authors around the world.
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