霍山石斛通过调节肠道微生物群和血清代谢物谱改善高脂诱导ApoE小鼠的动脉粥样硬化

IF 6.7 1区医学 Q1 CHEMISTRY, MEDICINAL

Phytomedicine Pub Date : 2025-06-18 DOI:10.1016/j.phymed.2025.156964

Shao-Jun Zheng , Xiao-Yan Gao , Xiao-Han Diao , Nai-Dong Chen

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引用次数: 0

摘要

背景：心血管疾病，特别是动脉粥样硬化（AS），仍然是导致死亡的主要原因，有效的治疗方法有限。霍山石斛是一种传统的中草药，具有良好的抗炎和抗氧化作用，但其对心血管的保护作用尚未得到充分的开发。目的探讨霍山石斛多糖（DHP）对AS的保护作用，并阐明其机制。方法建立ApoE（−/−）小鼠AS模型，灌胃不同浓度DHP。14周后，采集血清和粪便样本。评估DHP对血脂谱、主动脉斑块、基质金属蛋白酶（MMP-2和MMP-9）以及Nrf2/HO-1通路的影响。此外，对粪便样本进行宏基因组测序和血清非靶向代谢组学，并探讨这些发现之间的相关性。结果dhp改善小鼠血脂，降低活性氧（ROS）和丙二醛（MDA）水平，增强超氧化物歧化酶（SOD）活性。稳定主动脉斑块，抑制MMP-2和MMP-9过表达，激活Nrf2/HO-1通路。DHP还能促进肠道菌群平衡，增加Mucispirillum、双歧杆菌和Faecalibaculum，减少Desulfovibrionaceae和真菌。代谢组学揭示了代谢物的改变，包括牛磺酸脱氧胆酸和熊胆酸，影响氨基酸、甘油磷脂和胆汁酸代谢。结论dhp能有效降低血脂水平，稳定主动脉斑块，恢复肠道菌群平衡，纠正代谢紊乱，从而抑制动脉粥样硬化的进展。本研究结果为临床上应用DHP预防和治疗AS提供了科学依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Dendrobium huoshanense improves atherosclerosis in high-fat-induced ApoE mice by regulating gut microbiota and serum metabolite profiles

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Dendrobium huoshanense improves atherosclerosis in high-fat-induced ApoE mice by regulating gut microbiota and serum metabolite profiles

Background

Cardiovascular diseases, particularly atherosclerosis (AS), remain leading causes of mortality, with limited effective treatments available. Dendrobium huoshanense, a traditional medicinal herb, has shown promising anti-inflammatory and antioxidant effects, but its cardiovascular protective potential remains underexplored.

Purpose

This study aimed to explore the protective effects of Dendrobium huoshanense polysaccharides (DHP) against AS and elucidate the underlying mechanisms involved.

Methods

An ApoE(−/−) mice model of AS was established, and DHP was administered at different concentrations via gavage. After 14 weeks, serum and fecal samples were collected. The effects of DHP on lipid profiles, aortic plaques, matrix metalloproteinases (MMP-2 and MMP-9), and the Nrf2/HO-1 pathway were assessed. Additionally, metagenomic sequencing of fecal samples and untargeted metabolomics of serum were conducted and correlations between these findings were explored.

Results

DHP improved lipid profiles, reduced reactive oxygen species (ROS) and malondialdehyde (MDA) levels, and enhanced superoxide dismutase (SOD) activity. It stabilized aortic plaques, suppressed MMP-2 and MMP-9 overexpression, and activated the Nrf2/HO-1 pathway. DHP also promoted gut microbiota balance, increasing Mucispirillum, Bifidobacterium, and Faecalibaculum while decreasing Desulfovibrionaceae and Eubacterium. Metabolomics revealed alterations in metabolites, including taurochenodeoxycholic acid and ursocolic acid, influencing amino acid, glycerophospholipid, and bile acid metabolism.

Conclusions

DHP effectively lowers lipid levels, stabilizes aortic plaques, restores gut microbiota balance, and corrects metabolic disturbances, thereby inhibiting the progression of atherosclerosis. These findings provide a scientific basis for the clinical use of DHP in AS prevention and treatment.

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来源期刊

Phytomedicine 医学-药学

CiteScore

10.30

自引率

5.10%

发文量

670

审稿时长

91 days

期刊介绍： Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.