菊花多糖：超滤分离及抗氧化抗炎作用的优化。

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-06-18 DOI:10.1016/j.ijbiomac.2025.145383

Hua Hang , Dongsheng Du , Yue Xing, Ke Li, Runze Chen, Yanru Sun, Changbao Wang

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引用次数: 0

摘要

摘要黄山公菊粗多糖（PCMC）因其具有抗氧化和抗炎活性，在医药和食品领域受到广泛关注。本研究旨在利用超滤技术对其纯化浓缩工艺进行优化，并利用超滤技术对生物大分子溶质进行纯化浓缩。以PCM-C为原料，采用单因素试验和响应面法（RSM）研究了压力、温度和超滤浓度对膜通量的影响。建立的回归模型具有明显的典型，得到最佳超滤分离条件为：0.27 MPa, 51°c，材料浓度为2.9 mg/mL。在最佳条件下，PCM-C溶液的膜通量为39.26 ± 0.1 L/m2·h。通过超滤分离得到两种多糖（PCM-I和PCM-II）。PCMC、PCM-I和PCM-II的傅里叶变换红外（FT-IR）光谱特性确定了多糖的显著吸收峰。PCMs微观结构的SEM图像显示了非晶态粉末的典型特性。PCM-II抗氧化能力最强，分别为76.8% %、89.6% %、78.3% %和77.2% %。此外，在低浓度25 μg/mL时，PCM-I抑制NO释放炎症相关基因的表达，IL - 1β和IL-6表达量分别为87.78 %和52.17 %，TNF-α表达量为33.02 %，表明PCM-I对RAW264.7细胞生长具有较强的抗炎作用。因此，这些结果表明，pcm - 1可以作为潜在的生物功能成分用于制药和食品工业。

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Polysaccharides from Chrysanthemum morifolium: Optimization of ultrafiltration isolation and anti-oxidant and anti-inflammatory effects

Chrysanthemum morifolium (Huangshan Gongju) crude polysaccharide (PCMC) has attracted considerable attention in the pharmaceutical and food industries due to its potential anti-oxidant and anti-inflammatory activities. This study aims to optimize its purification and concentration process using ultrafiltration technology, which was used to purify and concentrate the bio-macromolecular solutes. Employing PCM-C as the rough material, single-factor trials and response surface methodology (RSM) were used to estimate the effects of pressure, temperature and concentration for ultrafiltration on membrane fluxes. The built regression model was obviously typical, and the optimum conditions of ultrafiltration isolation were obtained as follows: 0.27 MPa, 51°Cand material concentration of 2.9 mg/mL. A membrane flux of PCM-C solution attained 39.26 ± 0.1 L/m²·h at the optimum condition. Two polysaccharides (PCM-I and PCM-II) obtained from ultrafiltration isolation. Fourier transform infrared (FT-IR) spectroscopy properties of PCMs (including PCMC, PCM-I and PCM-II) ascertained the significant absorption peaks of polysaccharides. The SEM images of PCMs microstructure illustrated typical properties of amorphous powders. The anti-oxidant activity of PCMs contained DPPH scavenging radicals, hydroxyl radicals, ABTS scavenging radicals and reducing power discovered that PCM-II possessed the maximal anti-oxidant capacity values for 76.8 %, 89.6 %, 78.3 % and 77.2 %, respectively. Furthermore, PCM-I inhibited expression of inflammation-related genes for NO release at lower concentration of 25 μg/mL, IL − 1β and IL-6 expression levels of 87.78 % and 52.17 %, and TNF-α expression level of 33.02 %, which showed that PCM-I had relatively stronger anti-inflammatory effects on RAW264.7 cell growth. Thus, the outcomes exhibited that PCM-I could serve as the potential bio-functional component for the pharmaceutical and food industry.

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.