Romy J Veersma, Corentin Lannuzel, Walter J J Gerrits, Sonja de Vries, Gijs van Erven, Mirjam A Kabel
{"title":"纤维饲料中木质素对猪体内消化率和转运标志的影响。","authors":"Romy J Veersma, Corentin Lannuzel, Walter J J Gerrits, Sonja de Vries, Gijs van Erven, Mirjam A Kabel","doi":"10.1021/acsagscitech.4c00752","DOIUrl":null,"url":null,"abstract":"<p><p>To assess the nutritional value of fibrous diets, external metal oxide-based markers are commonly used. Given the increasing restrictions on the use of these conventional markers, we investigated the inertness of lignin in pigs and correspondingly its potential as an internal marker. Hereto, feces and digesta were collected from pigs fed a diet containing wheat straw as sole lignin source, and lignin was quantified by <sup>13</sup>C-IS py-GC-MS. Combined with detailed HSQC NMR and size-exclusion chromatography structural analysis, we revealed that lignin was recovered unmodified in feces. Comparison of lignin with TiO<sub>2</sub> showed similar (<i>P</i> > 0.15) digestibility values for nitrogen (82.6 vs 80.2%) and fat (93.3 vs 92.5%), and a limited difference for dry matter (77.7 vs 74.1%, <i>P</i> = 0.01). Comparison of lignin with chromium-mordanted straw furthermore showed highly similar (<i>P</i> = 0.8) mean retention times of fibrous particles in the stomach. We thus confirm the potential of intrinsic lignin as a multifunctional marker for feed digestibility and digesta transit in pigs.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"1025-1033"},"PeriodicalIF":2.3000,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12175119/pdf/","citationCount":"0","resultStr":"{\"title\":\"Lignin in Fibrous Feed as an Internal Digestibility and Transit Marker in Pigs.\",\"authors\":\"Romy J Veersma, Corentin Lannuzel, Walter J J Gerrits, Sonja de Vries, Gijs van Erven, Mirjam A Kabel\",\"doi\":\"10.1021/acsagscitech.4c00752\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>To assess the nutritional value of fibrous diets, external metal oxide-based markers are commonly used. Given the increasing restrictions on the use of these conventional markers, we investigated the inertness of lignin in pigs and correspondingly its potential as an internal marker. Hereto, feces and digesta were collected from pigs fed a diet containing wheat straw as sole lignin source, and lignin was quantified by <sup>13</sup>C-IS py-GC-MS. Combined with detailed HSQC NMR and size-exclusion chromatography structural analysis, we revealed that lignin was recovered unmodified in feces. Comparison of lignin with TiO<sub>2</sub> showed similar (<i>P</i> > 0.15) digestibility values for nitrogen (82.6 vs 80.2%) and fat (93.3 vs 92.5%), and a limited difference for dry matter (77.7 vs 74.1%, <i>P</i> = 0.01). Comparison of lignin with chromium-mordanted straw furthermore showed highly similar (<i>P</i> = 0.8) mean retention times of fibrous particles in the stomach. We thus confirm the potential of intrinsic lignin as a multifunctional marker for feed digestibility and digesta transit in pigs.</p>\",\"PeriodicalId\":93846,\"journal\":{\"name\":\"ACS agricultural science & technology\",\"volume\":\"5 6\",\"pages\":\"1025-1033\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-04-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12175119/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS agricultural science & technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1021/acsagscitech.4c00752\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/6/16 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS agricultural science & technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1021/acsagscitech.4c00752","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/16 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
为了评估纤维膳食的营养价值，通常使用外部金属氧化物标记物。鉴于对使用这些常规标记物的限制越来越多，我们研究了木质素在猪体内的惰性及其作为内部标记物的潜力。本试验收集以麦秸为唯一木质素源饲粮的猪的粪便和食糜，采用13C-IS ppy - gc - ms法对木质素进行定量测定。结合详细的HSQC核磁共振和排粒径层析结构分析，我们发现木质素在粪便中未经修饰地回收。木质素和TiO2对氮（82.6 vs 80.2%）和脂肪（93.3 vs 92.5%）的消化率相近（P < 0.05），对干物质（77.7 vs 74.1%）的消化率差异有限（P = 0.01）。此外，木质素与铬浸染秸秆相比，纤维颗粒在胃中的平均滞留时间非常相似（P = 0.8）。因此，我们证实了内在木质素作为猪饲料消化率和食糜转运的多功能标记物的潜力。
Lignin in Fibrous Feed as an Internal Digestibility and Transit Marker in Pigs.
To assess the nutritional value of fibrous diets, external metal oxide-based markers are commonly used. Given the increasing restrictions on the use of these conventional markers, we investigated the inertness of lignin in pigs and correspondingly its potential as an internal marker. Hereto, feces and digesta were collected from pigs fed a diet containing wheat straw as sole lignin source, and lignin was quantified by 13C-IS py-GC-MS. Combined with detailed HSQC NMR and size-exclusion chromatography structural analysis, we revealed that lignin was recovered unmodified in feces. Comparison of lignin with TiO2 showed similar (P > 0.15) digestibility values for nitrogen (82.6 vs 80.2%) and fat (93.3 vs 92.5%), and a limited difference for dry matter (77.7 vs 74.1%, P = 0.01). Comparison of lignin with chromium-mordanted straw furthermore showed highly similar (P = 0.8) mean retention times of fibrous particles in the stomach. We thus confirm the potential of intrinsic lignin as a multifunctional marker for feed digestibility and digesta transit in pigs.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
