Xia Wang, Jianjun Dong, Ying Wang, Mingyan Gao, Fengyan Hou, Jingyu Wang, Ye Li, Zuobin Wang
{"title":"粘弹性及其对DNA分子操作的影响。","authors":"Xia Wang, Jianjun Dong, Ying Wang, Mingyan Gao, Fengyan Hou, Jingyu Wang, Ye Li, Zuobin Wang","doi":"10.1002/jemt.70004","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>The viscoelasticity of DNA molecules in the air were investigated using atomic force microscopy (AFM). For the individual DNA, compared to the randomly-coiled DNA, the stretched DNA showed smaller viscoelasticity, measured in the AFM quantitative imaging (QI) mode. The manipulation of DNA was performed, and it was influenced by its viscoelasticity. Compared to the stretched DNA, significant highlighted accumulations of DNA and lateral dragging tails were more obvious for the randomly-coiled DNA, which was caused by the elastic recoil and adhesion effects. In addition, the viscoelasticity of DNA aggregates composed of multiple DNA strands were measured. Compared to the individual DNA, they showed larger viscoelasticity. This study serves as a reference for the studies of DNA viscoelasticity-related behaviors in the air and has potential in gene knockout and targeted gene editing.</p>\n </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 10","pages":"2733-2743"},"PeriodicalIF":2.1000,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Viscoelasticity and Its Influence on the Manipulation of DNA Molecules\",\"authors\":\"Xia Wang, Jianjun Dong, Ying Wang, Mingyan Gao, Fengyan Hou, Jingyu Wang, Ye Li, Zuobin Wang\",\"doi\":\"10.1002/jemt.70004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>The viscoelasticity of DNA molecules in the air were investigated using atomic force microscopy (AFM). For the individual DNA, compared to the randomly-coiled DNA, the stretched DNA showed smaller viscoelasticity, measured in the AFM quantitative imaging (QI) mode. The manipulation of DNA was performed, and it was influenced by its viscoelasticity. Compared to the stretched DNA, significant highlighted accumulations of DNA and lateral dragging tails were more obvious for the randomly-coiled DNA, which was caused by the elastic recoil and adhesion effects. In addition, the viscoelasticity of DNA aggregates composed of multiple DNA strands were measured. Compared to the individual DNA, they showed larger viscoelasticity. This study serves as a reference for the studies of DNA viscoelasticity-related behaviors in the air and has potential in gene knockout and targeted gene editing.</p>\\n </div>\",\"PeriodicalId\":18684,\"journal\":{\"name\":\"Microscopy Research and Technique\",\"volume\":\"88 10\",\"pages\":\"2733-2743\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2025-06-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microscopy Research and Technique\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jemt.70004\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ANATOMY & MORPHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microscopy Research and Technique","FirstCategoryId":"5","ListUrlMain":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jemt.70004","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}
Viscoelasticity and Its Influence on the Manipulation of DNA Molecules
The viscoelasticity of DNA molecules in the air were investigated using atomic force microscopy (AFM). For the individual DNA, compared to the randomly-coiled DNA, the stretched DNA showed smaller viscoelasticity, measured in the AFM quantitative imaging (QI) mode. The manipulation of DNA was performed, and it was influenced by its viscoelasticity. Compared to the stretched DNA, significant highlighted accumulations of DNA and lateral dragging tails were more obvious for the randomly-coiled DNA, which was caused by the elastic recoil and adhesion effects. In addition, the viscoelasticity of DNA aggregates composed of multiple DNA strands were measured. Compared to the individual DNA, they showed larger viscoelasticity. This study serves as a reference for the studies of DNA viscoelasticity-related behaviors in the air and has potential in gene knockout and targeted gene editing.
期刊介绍：
Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.
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