Rachel E Bosserman, Nicole J Tarlton, Kelly Alvarado, Brittany Roemmich, Melanie L Yarbrough
{"title":"VITEK MS PRIME和Bruker Biotyper MALDI-TOF质谱系统的性能和工作流程比较。","authors":"Rachel E Bosserman, Nicole J Tarlton, Kelly Alvarado, Brittany Roemmich, Melanie L Yarbrough","doi":"10.1128/jcm.00211-25","DOIUrl":null,"url":null,"abstract":"<p><p>Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is widely used for rapid identification of bacteria and yeast in clinical labs. The VITEK MS PRIME (\"PRIME\") is the latest MALDI-TOF MS system from bioMérieux. This study evaluated PRIME's performance and workflow timing against the MALDI Biotyper CA System by Bruker (\"Biotyper\"). We compared a collection of 154 bacteria and yeast clinical isolates from various specimen types using three methods: Biotyper target with a toothpick, PRIME target with the PICKME nib, and PRIME target with a loop. Positive blood culture isolates were also analyzed using these methods, with high-experience (HEU, <i>n</i> = 300) and low-experience users (LEU, <i>n</i> = 50) after short (6-8 h) and routine (18-24 h) incubation on agar plates. Workflow timing, from sample processing to organism identification, was assessed to identify time savings. PRIME identified 96% (PICKME) and 95% (Loop) of challenge isolates to genus level, compared with Biotyper at 99%. Short incubation of positive blood culture isolates demonstrated similar species-level identification rates across all methods (Biotyper: 84%, PRIME PICKME: 80%, PRIME Loop: 81%), although more repeats were needed compared with routine incubation. No difference in species identification occurred between users for any method at short incubation (89%-91%, HEU, vs 79%-85% LEU). Single-target process times were comparable for all methods (55-59 min), whereas PRIME methods had shorter hands-on times for analysis of multiple targets (Biotyper: 53 min, PRIME PICKME: 39 min, PRIME Loop: 40 min). These findings highlight the comparable performance of the PRIME and Biotyper systems while demonstrating the potential for time savings with PRIME workflows, particularly in high-throughput settings.IMPORTANCEThis study provides a critical evaluation of the new VITEK MS PRIME MALDI-TOF MS system, comparing its performance and workflow efficiency against the Bruker MALDI Biotyper. The study investigates the success rate of isolate identification from short incubation of positive blood cultures, illustrating the utility of this technique for downstream workflows such as faster reporting of results for patient management and isolate identification for interpretation of rapid phenotypic AST. Analysis of different workflows demonstrated areas for potential time savings, particularly in high-throughput settings. These findings highlight the importance of optimizing MALDI-TOF MS workflows in the era of workforce shortages and lab centralization to enhance rapid pathogen identification and improve patient care.</p>","PeriodicalId":15511,"journal":{"name":"Journal of Clinical Microbiology","volume":" ","pages":"e0021125"},"PeriodicalIF":5.4000,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12345239/pdf/","citationCount":"0","resultStr":"{\"title\":\"Performance and workflow comparison of the VITEK MS PRIME and Bruker Biotyper MALDI-TOF MS systems.\",\"authors\":\"Rachel E Bosserman, Nicole J Tarlton, Kelly Alvarado, Brittany Roemmich, Melanie L Yarbrough\",\"doi\":\"10.1128/jcm.00211-25\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is widely used for rapid identification of bacteria and yeast in clinical labs. The VITEK MS PRIME (\\\"PRIME\\\") is the latest MALDI-TOF MS system from bioMérieux. This study evaluated PRIME's performance and workflow timing against the MALDI Biotyper CA System by Bruker (\\\"Biotyper\\\"). We compared a collection of 154 bacteria and yeast clinical isolates from various specimen types using three methods: Biotyper target with a toothpick, PRIME target with the PICKME nib, and PRIME target with a loop. Positive blood culture isolates were also analyzed using these methods, with high-experience (HEU, <i>n</i> = 300) and low-experience users (LEU, <i>n</i> = 50) after short (6-8 h) and routine (18-24 h) incubation on agar plates. Workflow timing, from sample processing to organism identification, was assessed to identify time savings. PRIME identified 96% (PICKME) and 95% (Loop) of challenge isolates to genus level, compared with Biotyper at 99%. Short incubation of positive blood culture isolates demonstrated similar species-level identification rates across all methods (Biotyper: 84%, PRIME PICKME: 80%, PRIME Loop: 81%), although more repeats were needed compared with routine incubation. No difference in species identification occurred between users for any method at short incubation (89%-91%, HEU, vs 79%-85% LEU). Single-target process times were comparable for all methods (55-59 min), whereas PRIME methods had shorter hands-on times for analysis of multiple targets (Biotyper: 53 min, PRIME PICKME: 39 min, PRIME Loop: 40 min). These findings highlight the comparable performance of the PRIME and Biotyper systems while demonstrating the potential for time savings with PRIME workflows, particularly in high-throughput settings.IMPORTANCEThis study provides a critical evaluation of the new VITEK MS PRIME MALDI-TOF MS system, comparing its performance and workflow efficiency against the Bruker MALDI Biotyper. The study investigates the success rate of isolate identification from short incubation of positive blood cultures, illustrating the utility of this technique for downstream workflows such as faster reporting of results for patient management and isolate identification for interpretation of rapid phenotypic AST. Analysis of different workflows demonstrated areas for potential time savings, particularly in high-throughput settings. These findings highlight the importance of optimizing MALDI-TOF MS workflows in the era of workforce shortages and lab centralization to enhance rapid pathogen identification and improve patient care.</p>\",\"PeriodicalId\":15511,\"journal\":{\"name\":\"Journal of Clinical Microbiology\",\"volume\":\" \",\"pages\":\"e0021125\"},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2025-08-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12345239/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Clinical Microbiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1128/jcm.00211-25\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/6/20 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Clinical Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1128/jcm.00211-25","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/20 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
基质辅助激光解吸电离飞行时间质谱法(MALDI-TOF MS)广泛应用于临床实验室中细菌和酵母菌的快速鉴定。VITEK MS PRIME(“PRIME”)是biomsamrieux最新的MALDI-TOF MS系统。本研究对比Bruker的MALDI Biotyper CA系统(“Biotyper”)评估了PRIME的性能和工作流程时间。我们使用三种方法对154种细菌和酵母菌临床分离物进行了比较:使用牙签的Biotyper靶标、使用PICKME笔尖的PRIME靶标和使用环的PRIME靶标。在琼脂平板上短暂(6-8小时)和常规(18-24小时)培养后,高经验(HEU, n = 300)和低经验(LEU, n = 50)的血培养阳性分离物也使用这些方法进行分析。从样品处理到生物体鉴定的工作流程时间被评估以确定节省的时间。与Biotyper的99%相比,PRIME在属水平鉴定出96% (PICKME)和95% (Loop)的攻毒分离菌。尽管与常规培养相比需要更多的重复,但所有方法对阳性血培养分离物的短期培养显示出相似的物种水平识别率(Biotyper: 84%, PRIME PICKME: 80%, PRIME Loop: 81%)。在短孵育期间,任何方法的使用者之间的物种鉴定均无差异(高浓铀89%-91% vs低浓铀79%-85%)。所有方法的单靶点处理时间都相当(55-59分钟),而PRIME方法的多靶点分析操作时间更短(Biotyper: 53分钟,PRIME PICKME: 39分钟,PRIME Loop: 40分钟)。这些发现突出了PRIME和Biotyper系统的可比性,同时展示了PRIME工作流程节省时间的潜力,特别是在高通量环境下。本研究对新的VITEK MS PRIME MALDI- tof质谱系统进行了关键评估,将其性能和工作流程效率与Bruker MALDI Biotyper进行了比较。该研究调查了从阳性血液培养物的短期孵育中分离物鉴定的成功率,说明了该技术在下游工作流程中的实用性,例如为患者管理更快地报告结果,以及为快速表型AST解释分离物鉴定。对不同工作流程的分析显示了潜在的节省时间的领域,特别是在高通量环境中。这些发现强调了在劳动力短缺和实验室集中的时代,优化MALDI-TOF MS工作流程对加强快速病原体鉴定和改善患者护理的重要性。
Performance and workflow comparison of the VITEK MS PRIME and Bruker Biotyper MALDI-TOF MS systems.
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is widely used for rapid identification of bacteria and yeast in clinical labs. The VITEK MS PRIME ("PRIME") is the latest MALDI-TOF MS system from bioMérieux. This study evaluated PRIME's performance and workflow timing against the MALDI Biotyper CA System by Bruker ("Biotyper"). We compared a collection of 154 bacteria and yeast clinical isolates from various specimen types using three methods: Biotyper target with a toothpick, PRIME target with the PICKME nib, and PRIME target with a loop. Positive blood culture isolates were also analyzed using these methods, with high-experience (HEU, n = 300) and low-experience users (LEU, n = 50) after short (6-8 h) and routine (18-24 h) incubation on agar plates. Workflow timing, from sample processing to organism identification, was assessed to identify time savings. PRIME identified 96% (PICKME) and 95% (Loop) of challenge isolates to genus level, compared with Biotyper at 99%. Short incubation of positive blood culture isolates demonstrated similar species-level identification rates across all methods (Biotyper: 84%, PRIME PICKME: 80%, PRIME Loop: 81%), although more repeats were needed compared with routine incubation. No difference in species identification occurred between users for any method at short incubation (89%-91%, HEU, vs 79%-85% LEU). Single-target process times were comparable for all methods (55-59 min), whereas PRIME methods had shorter hands-on times for analysis of multiple targets (Biotyper: 53 min, PRIME PICKME: 39 min, PRIME Loop: 40 min). These findings highlight the comparable performance of the PRIME and Biotyper systems while demonstrating the potential for time savings with PRIME workflows, particularly in high-throughput settings.IMPORTANCEThis study provides a critical evaluation of the new VITEK MS PRIME MALDI-TOF MS system, comparing its performance and workflow efficiency against the Bruker MALDI Biotyper. The study investigates the success rate of isolate identification from short incubation of positive blood cultures, illustrating the utility of this technique for downstream workflows such as faster reporting of results for patient management and isolate identification for interpretation of rapid phenotypic AST. Analysis of different workflows demonstrated areas for potential time savings, particularly in high-throughput settings. These findings highlight the importance of optimizing MALDI-TOF MS workflows in the era of workforce shortages and lab centralization to enhance rapid pathogen identification and improve patient care.
期刊介绍:
The Journal of Clinical Microbiology® disseminates the latest research concerning the laboratory diagnosis of human and animal infections, along with the laboratory's role in epidemiology and the management of infectious diseases.