在seqstudio™遗传分析仪上验证研究者argus y- 28qs试剂盒:用于genemapper id-x软件v1.6的新面板文件。

IF 2.3 3区 医学 Q1 MEDICINE, LEGAL
Giulia Soldati, Stefania Turrina, Francesco Ausania
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引用次数: 0

摘要

背景:研究者Argus Y-28 QS试剂盒是一种27个位点、6种染色的多重序列,包括6个快速突变的短串联重复序列(RM Y-STRs)。方法:将GeneMapper ID-X Software v1.6优化后的面板和bin集制作成新的文本文件。此外,使用SeqStudio™HID基因分析仪对调查员Argus Y-28 QS试剂盒进行了内部验证研究。结果:该研究的全部结果呈现在这里,包括精度、灵敏度、可重复性、口吃测定和减少体积分析的数据。文献记录了一项一致性研究和三份病例报告。最后,该试剂盒成功地用于进行能力测试。结论:研究者Argus Y-28 QS试剂盒在SeqStudio™HID基因分析仪上的检测结果令人满意。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Validation of the investigator argus y-28 qs kit on the seqstudio™ genetic analyzer for hid: a new panel file for genemapper id-x software v1.6.

Background: The Investigator Argus Y-28 QS Kit is a 27-locus, six-dye multiplex, which includes six rapidly mutating Short Tandem Repeats (RM Y-STRs).

Methods: A new text file containing the optimized panel and bin sets for GeneMapper ID-X Software v1.6 has been prepared. In addition, an internal validation study was conducted on the Investigator Argus Y-28 QS kit using the SeqStudio™ Genetic Analyzer for HID.

Results: The full results of that study are presented here, including data on precision, sensitivity, reproducibility, stutter determination, and reduction volume analysis. A concordance study and three case reports are documented. Finally, the kit was successfully used to conduct a proficiency test.

Conclusions: The obtained findings achieved a satisfactory performance of the Investigator Argus Y-28 QS Kit on the SeqStudio™ Genetic Analyzer for HID.

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来源期刊
CiteScore
5.80
自引率
9.50%
发文量
165
审稿时长
1 months
期刊介绍: The International Journal of Legal Medicine aims to improve the scientific resources used in the elucidation of crime and related forensic applications at a high level of evidential proof. The journal offers review articles tracing development in specific areas, with up-to-date analysis; original articles discussing significant recent research results; case reports describing interesting and exceptional examples; population data; letters to the editors; and technical notes, which appear in a section originally created for rapid publication of data in the dynamic field of DNA analysis.
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