来自晚期胚胎发生丰度（LEA）蛋白的短肽调节液-液相分离并阻止聚集

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical and biophysical research communications Pub Date : 2025-06-17 DOI:10.1016/j.bbrc.2025.152224

Yinghan Wu , Yusuke V. Morimoto , Shinya Ikeno

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引用次数: 0

摘要

来源于晚期胚胎发生丰度（LEA）蛋白的短肽非常重要，因为它们在体内通过液-液相分离（LLPS）发挥保护功能。令人兴奋的是，共聚焦荧光显微镜显示绿色荧光蛋白（GFP）在共表达LEA肽的大肠杆菌细胞中表现出异质性分布。在这一观察结果的指导下，我们进行了体外LLPS实验，发现LEA-II和LEA-K肽在联合LLPS模式下减弱了分离LLPS，同时增强了溶液的稳定性。这些数据支持了我们的假设，即LEA肽作为抑制蛋白质聚集的分子屏障，从而改善异源蛋白的表达。

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Short peptides from Late Embryogenesis Abundant (LEA) proteins tune liquid–liquid phase separation and prevent aggregation

Short peptides derived from Late Embryogenesis Abundant (LEA) proteins are critically important because they appear to exert their protective functions through liquid–liquid phase separation (LLPS) in vivo. Excitingly, confocal fluorescence microscopy revealed that green fluorescent protein (GFP) exhibited a heterogeneous intracellular distribution in Escherichia coli cells co-expressing LEA peptides. Guided by this observation, we conducted in vitro LLPS assays and found that the LEA-II and LEA-K peptides attenuated segregative LLPS while enhancing solution stability in the associative LLPS mode. These data support our hypothesis that LEA peptides function as molecular shields that suppress protein aggregation, thereby improving heterologous protein expression.

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来源期刊

Biochemical and biophysical research communications 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

1400

审稿时长

14 days

期刊介绍： Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination of timely and significant experimental results in diverse fields of biological research. The development of the "Breakthroughs and Views" section brings the minireview format to the journal, and issues often contain collections of special interest manuscripts. BBRC is published weekly (52 issues/year).Research Areas now include: Biochemistry; biophysics; cell biology; developmental biology; immunology ; molecular biology; neurobiology; plant biology and proteomics