根际是稻田中亚硝酸盐和硝酸盐依赖性甲烷厌氧氧化的热点

IF 7.3 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution Pub Date : 2025-06-20 DOI:10.1016/j.envpol.2025.126711

Yanping Wang, Yanan Bai, Lidong Shen, Bo Shang, Yang Ji, Xupeng Miao, Bingjie Ren, Yuling Yang, Wangting Yang, Zhaozhong Feng

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引用次数: 0

摘要

碳和氮循环的新组合通过亚硝酸盐和硝酸盐依赖的甲烷厌氧氧化（AOM）发生。然而，它们在水稻根际中的作用仍未被探索。研究了稻田根际土壤中亚硝酸盐和硝酸盐aom的贡献及其调控。结果表明，各施肥处理使根际土壤亚硝酸盐和硝酸盐aom活性分别比土壤体提高了132%和87%。根际土壤NC10细菌丰度（9.89×106-2.04×107 copy g-1干土）显著高于块状土壤（1.50-2.94×106 copy g-1干土）。高通量测序揭示了NC10细菌和ANME-2d古细菌在根际和块状土壤中的不同群落组成。此外，PLS-PM分析还发现，根际土壤和块状土壤的土壤性质存在显著差异，导致NC10细菌丰度的差异，最终导致亚硝酸盐- aom活性的差异。然而，硝酸盐- aom活性受土壤性质变化和ANME-2d古菌丰度的共同影响。本研究提供了开创性的证据，将根际定位为AOM的活跃区域，从而完善了AOM在水田中的作用评价。

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The rhizosphere as a hotspot for nitrite- and nitrate-dependent anaerobic oxidation of methane in paddy fields

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The rhizosphere as a hotspot for nitrite- and nitrate-dependent anaerobic oxidation of methane in paddy fields

A novel combination of carbon and nitrogen cycling occurs through nitrite- and nitrate-dependent anaerobic oxidation of methane (AOM). However, their role in the rice rhizosphere remains unexplored. This study investigates the contribution and regulation of nitrite- and nitrate-AOM in the rhizosphere soil of paddy fields. The results showed that nitrite- and nitrate-AOM activities were elevated in rhizosphere soil by 132 % and 87 %, respectively, compared to bulk soil across all fertilization treatments. Rhizosphere soil exhibited a significantly higher abundance of NC10 bacteria (9.89 × 10⁶–2.04 × 10⁷ copies g⁻¹ dry soil) than bulk soil (1.50–2.94 × 10⁶ copies g⁻¹ dry soil). High-throughput sequencing unveiled distinct community compositions of NC10 bacteria and ANME-2d archaea in rhizosphere and bulk soils. Furthermore, PLS-PM analysis identified that significant variations in soil properties between rhizosphere and bulk soils resulted in the difference of NC10 bacterial abundance, finally leading to the difference in nitrite-AOM activity. However, nitrate-AOM activity was collectively influenced by the variations in soil properties and the abundance of ANME-2d archaea. This study provides pioneering evidence, positioning the rhizosphere as an active region for AOM, thereby refining the evaluation of AOM's role in paddy fields.

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来源期刊

Environmental Pollution 环境科学-环境科学

CiteScore

16.00

自引率

6.70%

发文量

2082

审稿时长

2.9 months

期刊介绍： Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health. Subject areas include, but are not limited to: • Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies; • Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change; • Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects; • Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects; • Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest; • New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.