Kaikai Qin, Yirong Zhang, Xiangyu Zhang, Yi Yang, Xiao Kong, Chenglan Liu
{"title":"吡唑菌酯通过群体感应诱导大肠杆菌耐多药:一个新的视角","authors":"Kaikai Qin, Yirong Zhang, Xiangyu Zhang, Yi Yang, Xiao Kong, Chenglan Liu","doi":"10.1016/j.jhazmat.2025.139006","DOIUrl":null,"url":null,"abstract":"Antibiotic resistance seriously threatens to global public health, and non-antibiotic chemicals like pesticides can contribute to its development. Quorum sensing (QS) is an intercellular communication system that regulates group behavior and can potentially become a pathway for the development of antibiotic resistance. This study firstly discovered that exposure to pyraclostrobin at 0.5<!-- --> <!-- -->mg/L activated QS, resulting in antibiotic resistance in <em>Escherichia coli</em>, with minimum inhibitory concentrations (MICs) increasing by up to 128-fold against tested antibiotics. Mechanistically, the high expression of the <em>luxS</em> gene induced by pyraclostrobin stress increased the level of the QS signal molecule (AI-2), leading to enhanced QS in antibiotic-resistant bacteria (ARB), thereby upregulating the expression of multidrug efflux pump genes (<em>acrB</em> and <em>marA</em>) and downregulating the expression of outer membrane porin genes (<em>ompC</em> and <em>ompF</em>). Meanwhile, using a QS inhibitor also increased the strains’ antibiotic sensitivity. Additionally, pyraclostrobin exposure damaged cell membranes, induced oxidative stress, and caused gene mutations, further promoting multidrug resistance. Overall, the findings demonstrate that pyraclostrobin exposure can stimulate antibiotic resistance in <em>Escherichia coli</em> by activating QS and inducing gene mutations. Therefore, the rigorous application of fungicides is essential to retard the development of antibiotic resistance.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"607 1","pages":""},"PeriodicalIF":12.2000,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pyraclostrobin induces multi-antibiotic resistance in Escherichia coli via quorum sensing: A new perspective\",\"authors\":\"Kaikai Qin, Yirong Zhang, Xiangyu Zhang, Yi Yang, Xiao Kong, Chenglan Liu\",\"doi\":\"10.1016/j.jhazmat.2025.139006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Antibiotic resistance seriously threatens to global public health, and non-antibiotic chemicals like pesticides can contribute to its development. Quorum sensing (QS) is an intercellular communication system that regulates group behavior and can potentially become a pathway for the development of antibiotic resistance. This study firstly discovered that exposure to pyraclostrobin at 0.5<!-- --> <!-- -->mg/L activated QS, resulting in antibiotic resistance in <em>Escherichia coli</em>, with minimum inhibitory concentrations (MICs) increasing by up to 128-fold against tested antibiotics. Mechanistically, the high expression of the <em>luxS</em> gene induced by pyraclostrobin stress increased the level of the QS signal molecule (AI-2), leading to enhanced QS in antibiotic-resistant bacteria (ARB), thereby upregulating the expression of multidrug efflux pump genes (<em>acrB</em> and <em>marA</em>) and downregulating the expression of outer membrane porin genes (<em>ompC</em> and <em>ompF</em>). Meanwhile, using a QS inhibitor also increased the strains’ antibiotic sensitivity. Additionally, pyraclostrobin exposure damaged cell membranes, induced oxidative stress, and caused gene mutations, further promoting multidrug resistance. Overall, the findings demonstrate that pyraclostrobin exposure can stimulate antibiotic resistance in <em>Escherichia coli</em> by activating QS and inducing gene mutations. Therefore, the rigorous application of fungicides is essential to retard the development of antibiotic resistance.\",\"PeriodicalId\":361,\"journal\":{\"name\":\"Journal of Hazardous Materials\",\"volume\":\"607 1\",\"pages\":\"\"},\"PeriodicalIF\":12.2000,\"publicationDate\":\"2025-06-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Hazardous Materials\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jhazmat.2025.139006\",\"RegionNum\":1,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Hazardous Materials","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.jhazmat.2025.139006","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Pyraclostrobin induces multi-antibiotic resistance in Escherichia coli via quorum sensing: A new perspective
Antibiotic resistance seriously threatens to global public health, and non-antibiotic chemicals like pesticides can contribute to its development. Quorum sensing (QS) is an intercellular communication system that regulates group behavior and can potentially become a pathway for the development of antibiotic resistance. This study firstly discovered that exposure to pyraclostrobin at 0.5 mg/L activated QS, resulting in antibiotic resistance in Escherichia coli, with minimum inhibitory concentrations (MICs) increasing by up to 128-fold against tested antibiotics. Mechanistically, the high expression of the luxS gene induced by pyraclostrobin stress increased the level of the QS signal molecule (AI-2), leading to enhanced QS in antibiotic-resistant bacteria (ARB), thereby upregulating the expression of multidrug efflux pump genes (acrB and marA) and downregulating the expression of outer membrane porin genes (ompC and ompF). Meanwhile, using a QS inhibitor also increased the strains’ antibiotic sensitivity. Additionally, pyraclostrobin exposure damaged cell membranes, induced oxidative stress, and caused gene mutations, further promoting multidrug resistance. Overall, the findings demonstrate that pyraclostrobin exposure can stimulate antibiotic resistance in Escherichia coli by activating QS and inducing gene mutations. Therefore, the rigorous application of fungicides is essential to retard the development of antibiotic resistance.
期刊介绍:
The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.