F Laghari, H Zhang, C He, H Gong, J Zhang, Q Chang, J Bao, R Zhang
{"title":"白藜芦醇减轻鸡应激相关的法氏囊损伤：转录组学分析。","authors":"F Laghari, H Zhang, C He, H Gong, J Zhang, Q Chang, J Bao, R Zhang","doi":"10.1080/00071668.2025.2512376","DOIUrl":null,"url":null,"abstract":"<p><p>1. Resveratrol (RSV) is an antioxidant and anti-inflammatory plant polyphenol that is widely used for the prevention of stress-related diseases. Chronic unpredictable mild stress (CUMS) significantly affects the immune system of layers, leading to substantial losses in growth performance and welfare. The aim of this study was to explore the effect of a multi-stress environment under CUMS conditions on the bursa of Fabricius and any therapeutic effects.2. A total of 288 healthy, one-day-old layer chicks were divided into three groups: a healthy control, challenged with CUMS or CUMS+RSV. At 42 d of age, bursa of Fabricius samples were collected and analysed. Transmission electron microscopy (TEM), haematoxylin and eosin (H&E) staining, RNA-seq technology, qRT-PCR, Western blots, immunofluorescence and immunohistochemical staining were used to assess tissue damage and mRNA expression and enrichment pathways.3. The tissue damage under CUMS challenge was observed and RSV alleviated the degree of injury from the results of H&E staining and TEM. Transcriptome analysis identified a total of 631, 293 and 598 differentially expressed genes (DEG; log2 fold change > 1, <i>p</i> < 0.05) in the control <i>vs</i>. CUMS, CUMS <i>vs</i>. CUMS+RSV and control <i>vs</i>. CUMS+RSV group, respectively.4. Genes related to neuroactive ligand-receptor interaction were <i>NTSR1</i> and <i>GZMA</i> down-regulated while <i>KNG1</i>, <i>OPRL1</i> and <i>P2RY8</i> were up-regulated and calcium signalling pathway genes <i>CXCR4, PLCG2, PRKCB</i> and <i>CAMK2A</i> were up-regulated in the CUMS group compared to control group. However, <i>CXCR4, PLCG2</i> and <i>CAMK2A</i> were down-regulated, whereas <i>NTSR1</i> and <i>GZMA</i> were up-regulated genes in CUMS+RSV group compared to the CUMS group. Analyses of DEG was conducted based on the KEGG pathway and verified using qRT PCR and Western blots.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"1-16"},"PeriodicalIF":1.7000,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Resveratrol alleviates stress-associated bursal injury in chickens: a transcriptomic analysis.\",\"authors\":\"F Laghari, H Zhang, C He, H Gong, J Zhang, Q Chang, J Bao, R Zhang\",\"doi\":\"10.1080/00071668.2025.2512376\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>1. Resveratrol (RSV) is an antioxidant and anti-inflammatory plant polyphenol that is widely used for the prevention of stress-related diseases. Chronic unpredictable mild stress (CUMS) significantly affects the immune system of layers, leading to substantial losses in growth performance and welfare. The aim of this study was to explore the effect of a multi-stress environment under CUMS conditions on the bursa of Fabricius and any therapeutic effects.2. A total of 288 healthy, one-day-old layer chicks were divided into three groups: a healthy control, challenged with CUMS or CUMS+RSV. At 42 d of age, bursa of Fabricius samples were collected and analysed. Transmission electron microscopy (TEM), haematoxylin and eosin (H&E) staining, RNA-seq technology, qRT-PCR, Western blots, immunofluorescence and immunohistochemical staining were used to assess tissue damage and mRNA expression and enrichment pathways.3. The tissue damage under CUMS challenge was observed and RSV alleviated the degree of injury from the results of H&E staining and TEM. Transcriptome analysis identified a total of 631, 293 and 598 differentially expressed genes (DEG; log2 fold change > 1, <i>p</i> < 0.05) in the control <i>vs</i>. CUMS, CUMS <i>vs</i>. CUMS+RSV and control <i>vs</i>. CUMS+RSV group, respectively.4. Genes related to neuroactive ligand-receptor interaction were <i>NTSR1</i> and <i>GZMA</i> down-regulated while <i>KNG1</i>, <i>OPRL1</i> and <i>P2RY8</i> were up-regulated and calcium signalling pathway genes <i>CXCR4, PLCG2, PRKCB</i> and <i>CAMK2A</i> were up-regulated in the CUMS group compared to control group. However, <i>CXCR4, PLCG2</i> and <i>CAMK2A</i> were down-regulated, whereas <i>NTSR1</i> and <i>GZMA</i> were up-regulated genes in CUMS+RSV group compared to the CUMS group. 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Resveratrol alleviates stress-associated bursal injury in chickens: a transcriptomic analysis.
1. Resveratrol (RSV) is an antioxidant and anti-inflammatory plant polyphenol that is widely used for the prevention of stress-related diseases. Chronic unpredictable mild stress (CUMS) significantly affects the immune system of layers, leading to substantial losses in growth performance and welfare. The aim of this study was to explore the effect of a multi-stress environment under CUMS conditions on the bursa of Fabricius and any therapeutic effects.2. A total of 288 healthy, one-day-old layer chicks were divided into three groups: a healthy control, challenged with CUMS or CUMS+RSV. At 42 d of age, bursa of Fabricius samples were collected and analysed. Transmission electron microscopy (TEM), haematoxylin and eosin (H&E) staining, RNA-seq technology, qRT-PCR, Western blots, immunofluorescence and immunohistochemical staining were used to assess tissue damage and mRNA expression and enrichment pathways.3. The tissue damage under CUMS challenge was observed and RSV alleviated the degree of injury from the results of H&E staining and TEM. Transcriptome analysis identified a total of 631, 293 and 598 differentially expressed genes (DEG; log2 fold change > 1, p < 0.05) in the control vs. CUMS, CUMS vs. CUMS+RSV and control vs. CUMS+RSV group, respectively.4. Genes related to neuroactive ligand-receptor interaction were NTSR1 and GZMA down-regulated while KNG1, OPRL1 and P2RY8 were up-regulated and calcium signalling pathway genes CXCR4, PLCG2, PRKCB and CAMK2A were up-regulated in the CUMS group compared to control group. However, CXCR4, PLCG2 and CAMK2A were down-regulated, whereas NTSR1 and GZMA were up-regulated genes in CUMS+RSV group compared to the CUMS group. Analyses of DEG was conducted based on the KEGG pathway and verified using qRT PCR and Western blots.
期刊介绍：
From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .
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