在古细菌中,自组装的表层使细胞动力学沟变平,以帮助细胞分裂

IF 9.1 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Sherman Foo, Ido Caspy, Alice Cezanne, Tanmay A. M. Bharat, Buzz Baum
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引用次数: 0

摘要

表层或“s层”是蛋白质的二维晶格,它覆盖了广泛的古细菌和细菌,取代了细胞壁或荚膜多糖。s层被认为在化学和物理上将细胞与外部环境隔离方面起着重要作用。在这里,我们表明,当细胞生长时,通过SlaA单体填补晶格间隙的自组装过程,Sulfolobus acidocalarius s层的完整性得以维持。尽管这种物理上系在膜上的晶格可能会阻碍细胞分裂,但我们发现s层在细胞分裂时使膜变平,以加速escrt - iii依赖的细胞分裂,并且对于在机械应力条件下稳健、成功的细胞分裂很重要。综上所述,这些结果定义了控制s层自组装的规则,并展示了与底层膜耦合的柔性晶格涂层如何为细胞提供机械支撑,并有助于推动细胞形状快速和功能上的重要变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A self-assembling surface layer flattens the cytokinetic furrow to aid cell division in an archaeon
The surface layer or “S-layer” is a two-dimensional lattice of proteins that coats a wide range of archaea and bacteria in place of a cell wall or capsular polysaccharides. S-layers are thought to play an important role in chemically and physically insulating cells from the external environment. Here, we show that the integrity of the S-layer in Sulfolobus acidocaldarius is maintained as cells grow via a process of self-assembly as SlaA monomers fill gaps in the lattice. Although this lattice which is physically tethered to the membrane might be expected to hinder cell division, we show that the S-layer flattens the membrane at cytokinesis to accelerate ESCRT-III-dependent cell division—and is important for robust, successful cell divisions under conditions of mechanical stress. Taken together, these results define the rules governing S-layer self-assembly and show how a flexible lattice coat that is coupled to the underlying membrane can both provide a cell with mechanical support and help to drive rapid and functionally important changes in cell shape.
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来源期刊
CiteScore
19.00
自引率
0.90%
发文量
3575
审稿时长
2.5 months
期刊介绍: The Proceedings of the National Academy of Sciences (PNAS), a peer-reviewed journal of the National Academy of Sciences (NAS), serves as an authoritative source for high-impact, original research across the biological, physical, and social sciences. With a global scope, the journal welcomes submissions from researchers worldwide, making it an inclusive platform for advancing scientific knowledge.
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