宿主特异性血管内皮细胞对血管圆线虫的反应:红狐(Vulpes Vulpes)和家养狗的体外比较研究。

IF 4.8 2区 医学 Q2 IMMUNOLOGY
Frontiers in Cellular and Infection Microbiology Pub Date : 2025-06-03 eCollection Date: 2025-01-01 DOI:10.3389/fcimb.2025.1584663
Belinda Eisenhut, Aline Wittwer, Manuela Schnyder, Andreas W Oehm
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引用次数: 0

摘要

导语:由血管圆线虫引起的犬类血管圆线虫病会影响狗和红狐,狗会出现心肺和凝血功能障碍,而狐狸则大多是亚临床的。方法:本研究检测了这两个物种的主动脉内皮细胞对血管棘猴成虫全体细胞抗原提取物、第一期幼虫(L1)抗原和成虫排泄-分泌产物(ESP)的反应。通过反转录定量PCR (RT - qPCR)评估抗原暴露4和24小时后白细胞介素(IL) -6、-10和-33、细胞间粘附分子1 (ICAM-1)、血管粘附分子1 (VCAM-1)、内皮选择素(e -选择素)、血小板选择素(p -选择素)、血管内皮生长因子(VEGF)和单核细胞趋化蛋白1 (MCP-1)的差异基因表达。结果:ESP刺激4小时后,犬IL-10升高(1.8倍),狐IL-10降低(0.4倍)。两者IL-33均下降(分别为0.9倍和0.7倍)。VCAM-1在狐狸中上调更多(3.5倍,狗中上调1.2倍)。在成年抗原暴露后,p -选择素、ICAM-1和VCAM-1在狐狸细胞中比在狗细胞中增加更多(分别为1.4、1.7和3.1倍,分别为0.9、0.5和0.7倍)。L1抗原在狗中下调IL-10和MCP-1(分别为0.7和0.8倍),在狐狸中上调IL-10和MCP-1(分别为2.1和1.1倍)。在ESP刺激24小时后,狗的ICAM-1减少了0.8倍,而狐狸的ICAM-1增加了1.4倍。VCAM-1在狗中下调(0.6倍),而在狐狸中上调(12.9倍)。在这两种物种中,成年抗原暴露上调p -选择素,在狐狸中(4.8倍)高于狗(1.9倍)。ICAM-1在狗中下调0.8倍,而在狐狸中上调7.5倍。L1抗原刺激引起了物种之间最显著的差异:狗的IL-6上调(4.7倍)比狐狸的上调(1.2倍)更多。E-Selectin在狗中上调(12.8倍),而在狐狸中下调(0.2倍)。p -选择素在狗身上增加了10.0倍,在狐狸身上增加了1.7倍。ICAM-1在狗中下调(0.6倍),而在狐狸中上调(2.6倍),VCAM-1也上调(0.7倍和3.1倍)。在成年抗原暴露后,VEGF在狗中上调9.5倍,在L1抗原暴露后上调7.6倍,而在狐狸中则基本保持不变(分别为0.9倍和1.0倍)。讨论:这些发现证实了狐狸在接触a . vasorum后已经形成了调节免疫反应的机制,而狗表现出更高的促炎反应,导致严重的临床结果。宿主-寄生虫共同进化可能解释犬类血管线虫病的发病机制和临床表现的差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Host-specific vascular endothelial cell responses to Angiostrongylus vasorum: a comparative in vitro study in red foxes (Vulpes vulpes) and domestic dogs.

Introduction: Canine angiostrongylosis, caused by Angiostrongylus vasorum, affects dogs and red foxes, with dogs developing cardiopulmonary and coagulation disorders, while foxes remain mostly subclinical.

Methods: This study examined aortic endothelial cell responses from both species to A. vasorum adult full somatic antigen extracts, first-stage larval (L1) antigen, and adult excretory-secretory products (ESP). Differential gene expression of interleukins (IL) -6, -10, and -33, intercellular adhesion molecule 1 (ICAM-1), vascular adhesion molecule 1 (VCAM-1), endothelial selectin (E-selectin), platelet selectin (P-selectin), vascular endothelial growth factor (VEGF), and monocyte chemoattractant protein 1 (MCP-1) was assessed via reverse transcription quantitative PCR (RT qPCR) after four and 24 hours of antigen exposure.

Results: Four hours post ESP stimulation, IL-10 increased in dogs (1.8-fold) but decreased in foxes (0.4-fold). IL-33 declined in both, (0.9-fold vs. 0.7-fold, respectively). VCAM-1 was upregulated more in foxes (3.5-fold vs. 1.2 in dogs). Following adult antigen exposure, P-selectin, ICAM-1, and VCAM-1 increased in fox more than in dog cells (1.4, 1.7, and 3.1-fold vs. 0.9, 0.5, and 0.7-fold, respectively). L1 antigen downregulated IL-10 and MCP-1 in dogs (0.7 and 0.8-fold) but upregulated them in foxes (2.1 and 1.1-fold). Twenty-four hours after ESP stimulation, ICAM-1 decreased in dogs (0.8-fold) but increased in foxes (1.4-fold). VCAM-1 was downregulated in dogs (0.6-fold) but upregulated in foxes (12.9-fold). Adult antigen exposure upregulated P-selectin in both species, more in foxes (4.8-fold) than in dogs (1.9-fold). ICAM-1 was downregulated in dogs (0.8-fold) but upregulated 7.5-fold in foxes. L1 antigen stimulation caused the most substantial differences between species: IL-6 was upregulated more in dogs (4.7-fold) than foxes (1.2-fold). E-Selectin was upregulated in dogs (12.8-fold) but downregulated in foxes (0.2-fold). P-selectin increased more in dogs (10.0-fold) than in foxes (1.7-fold). ICAM-1 was downregulated in dogs (0.6-fold) but upregulated in foxes (2.6-fold), as was VCAM-1 (0.7-fold and 3.1-fold). VEGF was upregulated 9.5-fold in dogs after adult antigen exposure, and 7.6-fold after L1 antigen exposure, while it remained rather unchanged in foxes (0.9-fold and 1.0-fold, respectively).

Discussion: These findings corroborate that foxes have developed mechanisms for a regulated immune response following A. vasorum exposure, while dogs exhibit a higher pro-inflammatory reaction, contributing to severe clinical outcomes. Host-parasite co-evolution may explain differences in the pathogenesis and clinical presentation of canid angiostrongylosis.

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来源期刊
CiteScore
7.90
自引率
7.00%
发文量
1817
审稿时长
14 weeks
期刊介绍: Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.
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