{"title":"SYCP1和SYCP3在双头巨鲷杂交鱼中的分子特征及表达分析","authors":"Jia Xu, Yuandong Sun, Chang Wu, Xiaojuan Cui, Yujing Li, Zhongwei Hua, Yifei Zhang","doi":"10.1111/jwas.70030","DOIUrl":null,"url":null,"abstract":"<p><i>SYCP1</i> and <i>SYCP3</i> are essential testis-specific genes for centromere pairing during meiosis, as well as for spermatogenesis and fertility in male germ cells. However, it is still unclear regarding the expression patterns in the fertile reciprocal hybrid offspring of <i>Megalobrama amblycephala</i> (blunt snout bream, BSB, 2n = 48) × <i>Culter alburnus</i> (topmouth culter, TC, 2n = 48). This research elucidated the genetic and expression characteristics of <i>SYCP1</i> and <i>SYCP3</i> through molecular cloning, sequence alignment, Western blotting, and immunohistochemistry to assess their roles in both hybrids and parents. The findings revealed that <i>SYCP1</i> and <i>SYCP3</i> exhibited high homology between <i>M. amblycephala</i> and <i>C. alburnus</i>, with varying degrees of chimerism in the BT and TB hybrids. The expression level of <i>SYCP1</i> in these hybrids was intermediate between parents, while <i>SYCP3</i> was more similar to <i>M. amblycephala</i> and significantly different from <i>C. alburnus</i> (<i>p</i> < 0.05). Western blotting confirmed the normal expression of both SYCP1 and SYCP3 proteins in the hybrid offspring. Immunohistochemistry verified the significant presence of these proteins in the testes of mature hybrids. These findings suggested that BT and TB hybrids retained the stability of the <i>SYCP1</i> and <i>SYCP3</i> genes inherited from their heterozygous parental origins, supporting independent protein expression despite slight variations in the CDS structure. Our results demonstrate that the normal expression of key meiotic genes plays an important role in overcoming reproductive barriers in distant hybridization, which is of great significance for genetic breeding in fish.</p>","PeriodicalId":17284,"journal":{"name":"Journal of The World Aquaculture Society","volume":"56 3","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jwas.70030","citationCount":"0","resultStr":"{\"title\":\"Molecular characterization and expression analysis of SYCP1 and SYCP3 in hybrid fish derived from Megalobrama amblycephala × Culter alburnus\",\"authors\":\"Jia Xu, Yuandong Sun, Chang Wu, Xiaojuan Cui, Yujing Li, Zhongwei Hua, Yifei Zhang\",\"doi\":\"10.1111/jwas.70030\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>SYCP1</i> and <i>SYCP3</i> are essential testis-specific genes for centromere pairing during meiosis, as well as for spermatogenesis and fertility in male germ cells. However, it is still unclear regarding the expression patterns in the fertile reciprocal hybrid offspring of <i>Megalobrama amblycephala</i> (blunt snout bream, BSB, 2n = 48) × <i>Culter alburnus</i> (topmouth culter, TC, 2n = 48). This research elucidated the genetic and expression characteristics of <i>SYCP1</i> and <i>SYCP3</i> through molecular cloning, sequence alignment, Western blotting, and immunohistochemistry to assess their roles in both hybrids and parents. The findings revealed that <i>SYCP1</i> and <i>SYCP3</i> exhibited high homology between <i>M. amblycephala</i> and <i>C. alburnus</i>, with varying degrees of chimerism in the BT and TB hybrids. The expression level of <i>SYCP1</i> in these hybrids was intermediate between parents, while <i>SYCP3</i> was more similar to <i>M. amblycephala</i> and significantly different from <i>C. alburnus</i> (<i>p</i> < 0.05). Western blotting confirmed the normal expression of both SYCP1 and SYCP3 proteins in the hybrid offspring. Immunohistochemistry verified the significant presence of these proteins in the testes of mature hybrids. These findings suggested that BT and TB hybrids retained the stability of the <i>SYCP1</i> and <i>SYCP3</i> genes inherited from their heterozygous parental origins, supporting independent protein expression despite slight variations in the CDS structure. Our results demonstrate that the normal expression of key meiotic genes plays an important role in overcoming reproductive barriers in distant hybridization, which is of great significance for genetic breeding in fish.</p>\",\"PeriodicalId\":17284,\"journal\":{\"name\":\"Journal of The World Aquaculture Society\",\"volume\":\"56 3\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-06-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jwas.70030\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of The World Aquaculture Society\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jwas.70030\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of The World Aquaculture Society","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jwas.70030","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FISHERIES","Score":null,"Total":0}
Molecular characterization and expression analysis of SYCP1 and SYCP3 in hybrid fish derived from Megalobrama amblycephala × Culter alburnus
SYCP1 and SYCP3 are essential testis-specific genes for centromere pairing during meiosis, as well as for spermatogenesis and fertility in male germ cells. However, it is still unclear regarding the expression patterns in the fertile reciprocal hybrid offspring of Megalobrama amblycephala (blunt snout bream, BSB, 2n = 48) × Culter alburnus (topmouth culter, TC, 2n = 48). This research elucidated the genetic and expression characteristics of SYCP1 and SYCP3 through molecular cloning, sequence alignment, Western blotting, and immunohistochemistry to assess their roles in both hybrids and parents. The findings revealed that SYCP1 and SYCP3 exhibited high homology between M. amblycephala and C. alburnus, with varying degrees of chimerism in the BT and TB hybrids. The expression level of SYCP1 in these hybrids was intermediate between parents, while SYCP3 was more similar to M. amblycephala and significantly different from C. alburnus (p < 0.05). Western blotting confirmed the normal expression of both SYCP1 and SYCP3 proteins in the hybrid offspring. Immunohistochemistry verified the significant presence of these proteins in the testes of mature hybrids. These findings suggested that BT and TB hybrids retained the stability of the SYCP1 and SYCP3 genes inherited from their heterozygous parental origins, supporting independent protein expression despite slight variations in the CDS structure. Our results demonstrate that the normal expression of key meiotic genes plays an important role in overcoming reproductive barriers in distant hybridization, which is of great significance for genetic breeding in fish.
期刊介绍:
The Journal of the World Aquaculture Society is an international scientific journal publishing original research on the culture of aquatic plants and animals including:
Nutrition;
Disease;
Genetics and breeding;
Physiology;
Environmental quality;
Culture systems engineering;
Husbandry practices;
Economics and marketing.