Dodecylmethylaminoethyl methacrylate inhibits the growth of Candida albicans and Enterococcus faecalis biofilm and the formation of osteoclast

Objectives

This study aimed to evaluate the antimicrobial ability on the dual-species biofilm formed by C. albicans and E. faecalis and osteoclysis inhibition of DMAEM.

Design

DMAEM monomer was used to evaluate the antmicrobial ability on the dual-species biofilm of C. albicans and E. faecalis by biomass quantification, Scanning electron microscope and confocal laser scanning microscopy scanning, and RT-qPCR. The biosafty and inhibition ability on the dual-species biofilm and osteoclysis of the experimental root canal sealers containing DMAEM was tested by biomass quantification, Cell Counting kit-8, tartrate-resistant acid phosphatase staining, flow cytometry analysis, and RT-qPCR.

Results

The biomass of the dual-species biofilm formed by C. albicans and E. faecalis was significantly decreased by 26.3–51.3 % under 8–256 μg/mL DMAEM. 8 μg/mL DMAEM could reduce E. faecalis’s survival rate to 77.4 % (p < 0.001) and down-regulated its virulence gene expression. Meanwhile, the mycelium count proportion of C. albicans in the dual-species biofilm was reduced from 50 % to 0 % (p < 0.0001). The experimental sealers with DMAEM content could also decrease the survival rate of C.albicans and E.faecalis in the dual-species biofilm. Moreover, the elution of experimental root canal sealers containing ≤ 2.5 % DMAEM was biosafe and improved 81.0 % (p < 0.05) osteoclastic inhibition compared with it of unmodified sealers at least.

Conclusions

The antimicrobial ability on the dual-species biofilm formed by C. albicans and E. faecalis and osteoclastic inhibition of DMAEM suggested its potential clinical application of DMAEM in the treatment of periapical inflammation.