Antioxidant and Anti-Acetylcholinesterase Activities of Three Lippia L. Species: A Chemometric Approach.

The aim of this study was to compare the antioxidant and anti-acetylcholinesterase (anti-AChE) activities of certain species from the Lippia genus, relating them to previously identified metabolites by chemometric calculations. The antioxidant potentials were determined by DPPH, ABTS, deoxyribose protection, β-carotene, iron chelation, and reduction. The AChE inhibition activity was also evaluated. The data were related through multivariate analyses. All extracts showed significant antioxidant activity, especially Lippia gracilis, with an IC₅₀ of 0.59 ± 0.08 µg/mL for Fe²⁺ chelation and also demonstrated the best AChE inhibition. Multivariate analyses proved the effectiveness of Lippia sidoides constituents in Fe²⁺ chelation, DPPH radical reduction, deoxyribose protection, and anti-AChE. It is concluded that the results demonstrate important activities of the extracts, and further studies are needed to confirm the correlations between Lippia secondary metabolites and their use in food preservation.