马齿苋种子的植物化学筛选，抗氧化和抗菌分析与硅分子对接见解

IF 3.5 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology Pub Date : 2025-06-18 DOI:10.1016/j.jgeb.2025.100516

Khursheed Ahmad Sheikh , Reyaz Hassan Mir , Mohammad Ovais Dar , Adil Farooq Wali , Insha Qadir , Sheeba Nazir , Mohammed Iqbal Zargar , Sirajunisa Talath , Sathvik B. Sridhar , Javedh Shareef , Mubashir Hussain Masoodi

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引用次数: 0

摘要

马齿苋（porulaca oleracea， PO）是一种分布于全球的一年生多肉草本植物，自古以来就被用作药用，素有“全球万灵药”的称号。目的对番石榴种子进行植物化学筛选、抗氧化和抗菌分析，包括甲醇提取物的GCMS分析和抗菌机制的分子对接。材料和方法对spo种子进行植物化学筛选，并通过DPPH、NO自由基清除和还原能力分析甲醇提取物。对细菌和真菌进行了抑菌活性测试，用GCMS鉴定了化合物。通过Autodock进行分子对接，靶向β -微管蛋白（5FNV）和ABC转运蛋白（6J9W）。结果甲醇提取物具有较强的抗氧化活性（IC50：对DPPH 125.2µg/ml，对NO 402.89µg/ml）和浓度依赖性还原能力。对酿酒酵母菌和降毒弧菌有较好的抑菌效果。GCMS鉴定出秋水仙碱、正癸酸和三环氧癸烷，其中秋水仙碱对蛋白质靶点具有较高的结合亲和力。结论秋水仙碱的结合亲和性支持了甲醇提取物的抗氧化和抗菌作用，证实了其药用潜力，值得进一步探索。

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Phytochemical screening, antioxidant, and antimicrobial analysis of Portulaca oleracea seeds with in-silico molecular docking insights

Background

Portulaca oleracea (PO), an annual succulent herb with global distribution, has been used medicinally since ancient times, earning the title “global panacea.”

Aim

This study aimed to perform phytochemical screening, antioxidant and antimicrobial analysis of PO seeds, including GCMS analysis of methanolic extract and molecular docking for antimicrobial mechanisms.

Materials and Methods

PO seeds underwent phytochemical screening and methanolic extract analysis via DPPH, NO radical scavenging, and reducing power assays. Antimicrobial activity was tested against bacteria and fungi, with GCMS identifying compounds. Molecular docking was conducted via Autodock vina, against targets beta-tubulin (5FNV) and ABC transporter (6J9W).

Results

Methanolic extract showed strong antioxidant activity (IC₅₀: 125.2 µg/ml for DPPH, 402.89 µg/ml for NO) and concentration-dependent reducing power. It was highly effective against Saccharomyces cerevisiae and Hypochrea viridescens. GCMS identified colchicine, n-decanoic acid, and triepoxydecane, with colchicine showing high binding affinity to protein targets.

Conclusion

The methanolic extract’s potent antioxidant and antimicrobial effects, supported by colchicine’s binding affinity, validate PO’s medicinal potential, suggesting further therapeutic exploration.

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来源期刊

Journal of Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

5.70

自引率

5.70%

发文量

159

审稿时长

16 weeks

期刊介绍： Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts