绿色合成大麻素负载金纳米粒子显示增强抗癌特性

IF 7.9 3区 材料科学 Q1 GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY
Anshuman Jakhmola , Farshad Moradi Kashkooli , Kevin Rod , Monika Lodyga , Jahangir (Jahan) Tavakkoli , Michael C. Kolios
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引用次数: 0

摘要

本研究探讨了大麻素负载到金纳米颗粒(AuNPs)上用于靶向癌症治疗的抗癌潜力。我们采用了多方面的方法来探索和设计这种纳米系统,包括它的合成、物理化学表征、稳定性评估以及在体外2D和3D模型中的抗癌功效评估。在这项研究中,我们证明了两种高度疏水的植物大麻素,如δ -9-四氢大麻酚(THC)和大麻二酚(CBD),可以用柠檬酸三钠和l-酪氨酸作为还原和稳定剂的一锅合成方案装载在aunp表面。l-酪氨酸在大麻素装载、稳定性和aunp的保质期中起着至关重要的作用。合成后,利用紫外可见光谱、动态光散射、暗场高光谱显微镜和电子显微镜对负载大麻素的纳米颗粒进行了表征。AuNPs的功能是作为一种支架,促进两种大麻素在癌细胞内的附着和运输,从而提高它们的生物利用度。使用高光谱显微镜确认AuNPs摄取。四氢大麻酚和大麻二酚在SK-BR-3人乳腺癌细胞株中的IC50值分别比纯分子水悬浮液低70.75%和37.04%。与纯大麻素的水悬浮液相比,这种方法更有效地诱导和增强癌细胞死亡。流式细胞术结果显示,这种增强的功效与细胞活力的下降有关,这归因于细胞凋亡。我们的发现为绿色设计和利用aunp将大麻素有效地输送到细胞中提供了重要的一步。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Green synthesis of cannabinoids loaded gold nanoparticles displaying enhanced anti-cancer properties
This study investigates the anti-cancer potential of cannabinoids loaded onto gold nanoparticles (AuNPs) for targeted cancer treatment. We adopted a multifaceted approach to explore and design this nano system, encompassing its synthesis, physicochemical characterization, stability assessment, and evaluation of anticancer efficacy in 2D and 3D in vitro models. In this research, we have demonstrated that two highly hydrophobic phytocannabinoids like delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD), can be loaded on the surface of AuNPs with a one-pot synthesis protocol using trisodium citrate and l-tyrosine as a reducing and stabilizing agents. l-tyrosine plays a crucial role in cannabinoid loading, stability, and shelf life of the AuNPs. After synthesis, the cannabinoid-loaded nanoparticles were characterized with UV–vis spectroscopy, dynamic light scattering (DLS), dark field hyperspectral microscopy, and electron microscopy. The AuNPs function as a scaffold for the attachment and enhanced transport of both cannabinoids inside cancer cells, thus increasing their bioavailability. Hyperspectral microscopy was used to confirm AuNPs uptake. IC50 values in SK-BR-3 human breast cancer cell line for both THC and CBD loaded onto AuNPs were lower by 70.75 % and 37.04 % than those of the aqueous suspension of pure molecules. Compared to the aqueous suspension of pure cannabinoids, this approach induced and enhanced cancer cell death more efficiently. This enhanced efficacy was associated with a decline in cell viability, which is attributed to apoptosis, as indicated by flow cytometry results. Our findings offer a significant step towards the green design and utilization of AuNPs to deliver cannabinoids into cells efficiently.
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来源期刊
CiteScore
5.80
自引率
6.40%
发文量
174
审稿时长
32 days
期刊介绍: Materials Today Sustainability is a multi-disciplinary journal covering all aspects of sustainability through materials science. With a rapidly increasing population with growing demands, materials science has emerged as a critical discipline toward protecting of the environment and ensuring the long term survival of future generations.
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