PFP@SiO2作为液相色谱固定相的简便绿色合成及其在药物分析中的应用

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B Pub Date : 2025-06-17 DOI:10.1016/j.jchromb.2025.124706

Xuerui Zhang , Shuo Wang , Yixin Ren , Xiaoting Fu , Xiaodong Bi , Xia Wang , Ru-Song Zhao

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引用次数: 0

摘要

五氟苯基（PFP）功能化固定相由于其独特的疏水性和亲氟亲和性，在分离科学中受到越来越多的关注。如何用绿色化学的方法有效地引入PFP基团已成为一个重要的问题。为制备PFP@SiO2色谱柱提供了一条简便、绿色的合成路线。系统探讨了酚酸类、类黄酮类、抗病毒药物等药物分子的液相色谱性能。结果表明：PFP@SiO2柱背压低，耐溶剂性好，受溶剂效应影响较小；在梯度洗脱模式下，该柱对复杂天然产物（山地血提取物）也具有较高的分离效率和良好的定量能力，符合现行标准和法规的要求。本研究为制备高性能的PFP@SiO2色谱柱提供了一种简便的绿色合成方法，并为生物医学研究提供了有力的工具。

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Facile and green synthesis of PFP@SiO2 as stationary phase for liquid chromatography and its application for pharmaceutical analysis

The pentafluorophenyl (PFP)-functionalized stationary phases, due to their unique hydrophobicity and fluorophilic affinity, have received increasing attention in separation science. How to introduce PFP group effectively with a green chemistry approach has become an important issue. This work provided a facile and green synthetic route for preparing PFP@SiO₂ column. The liquid chromatography (LC) performance towards pharmaceutical molecules such as phenolic acids, flavonoids, and antiviral drugs was systematically explored. The results showed the PFP@SiO₂ column possessed a low back pressure and good solvent endurance (less affected by solvent effect). Under gradient elution mode, the column also showed a higher separation efficiency and good quantitative capacity towards complex natural product (Sanguisorbae Radix Carbonisatum extract), which met the requirements of current standards and regulations. This work provides a facile green synthesis approach for preparing highly-performed PFP@SiO₂ column for pharmaceutical applications, and a powerful tool for biomedicine.

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来源期刊

Journal of Chromatography B 医学-分析化学

CiteScore

5.60

自引率

3.30%

发文量

306

审稿时长

44 days

期刊介绍： The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.