Gui-Liu Yan , Dan Dai , Qiang Zi , Fu-Mei Zhang , Yun Li
{"title":"甘草素对脑卒中后抑郁大鼠海马BDNF、Bax、Bcl-2表达的影响","authors":"Gui-Liu Yan , Dan Dai , Qiang Zi , Fu-Mei Zhang , Yun Li","doi":"10.1016/j.cccb.2025.100388","DOIUrl":null,"url":null,"abstract":"<div><h3>Aims</h3><div>The study intended to explore the therapeutic effect of liquiritin on PSD rats and its role in the pathogenesis of PSD.</div></div><div><h3>Methods</h3><div>The stroke model was established via middle cerebral artery occlusion, and the PSD model was created using chronic unpredictable mild stress combined with isolated feeding. The expressions of BDNF, Bax, and Bcl-2 proteins in the hippocampus of rats were detected using Western blot and immunofluorescence staining after 6 weeks of modeling.</div></div><div><h3>Results</h3><div>The weight, sucrose consumption and activity of the PSD rats decreased (<em>P</em> < 0.05) compared with the normal control and stroke groups. On the contrary, the weights of the liquiritin and escitalopram groups increased and their sucrose consumption and activity increased in the open-field test (<em>P</em> < 0.05) compared with the PSD and normal saline (NS) groups.The result of immunofluorescence staining and western-blot showed that BDNF and Bcl-2 increased in the liquiritin group and Bax increased significantly in the stroke and PSD groups (<em>P</em> < 0.05).</div></div><div><h3>Conclusions</h3><div>Liquiritin is capable of inhibiting neuronal apoptosis in the hippocampus of PSD rats to improve depression symptoms. This improvement may be achieved by reducing the expression of Bax and increasing the expressions of Bcl-2 and BDNF in the hippocampus of PSD rats.</div></div>","PeriodicalId":72549,"journal":{"name":"Cerebral circulation - cognition and behavior","volume":"9 ","pages":"Article 100388"},"PeriodicalIF":2.8000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of liquiritin on the expression of BDNF, Bax, and Bcl-2 in the hippocampus of post-stroke depression rats\",\"authors\":\"Gui-Liu Yan , Dan Dai , Qiang Zi , Fu-Mei Zhang , Yun Li\",\"doi\":\"10.1016/j.cccb.2025.100388\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Aims</h3><div>The study intended to explore the therapeutic effect of liquiritin on PSD rats and its role in the pathogenesis of PSD.</div></div><div><h3>Methods</h3><div>The stroke model was established via middle cerebral artery occlusion, and the PSD model was created using chronic unpredictable mild stress combined with isolated feeding. The expressions of BDNF, Bax, and Bcl-2 proteins in the hippocampus of rats were detected using Western blot and immunofluorescence staining after 6 weeks of modeling.</div></div><div><h3>Results</h3><div>The weight, sucrose consumption and activity of the PSD rats decreased (<em>P</em> < 0.05) compared with the normal control and stroke groups. On the contrary, the weights of the liquiritin and escitalopram groups increased and their sucrose consumption and activity increased in the open-field test (<em>P</em> < 0.05) compared with the PSD and normal saline (NS) groups.The result of immunofluorescence staining and western-blot showed that BDNF and Bcl-2 increased in the liquiritin group and Bax increased significantly in the stroke and PSD groups (<em>P</em> < 0.05).</div></div><div><h3>Conclusions</h3><div>Liquiritin is capable of inhibiting neuronal apoptosis in the hippocampus of PSD rats to improve depression symptoms. This improvement may be achieved by reducing the expression of Bax and increasing the expressions of Bcl-2 and BDNF in the hippocampus of PSD rats.</div></div>\",\"PeriodicalId\":72549,\"journal\":{\"name\":\"Cerebral circulation - cognition and behavior\",\"volume\":\"9 \",\"pages\":\"Article 100388\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cerebral circulation - cognition and behavior\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666245025000121\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CLINICAL NEUROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cerebral circulation - cognition and behavior","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666245025000121","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CLINICAL NEUROLOGY","Score":null,"Total":0}
Effect of liquiritin on the expression of BDNF, Bax, and Bcl-2 in the hippocampus of post-stroke depression rats
Aims
The study intended to explore the therapeutic effect of liquiritin on PSD rats and its role in the pathogenesis of PSD.
Methods
The stroke model was established via middle cerebral artery occlusion, and the PSD model was created using chronic unpredictable mild stress combined with isolated feeding. The expressions of BDNF, Bax, and Bcl-2 proteins in the hippocampus of rats were detected using Western blot and immunofluorescence staining after 6 weeks of modeling.
Results
The weight, sucrose consumption and activity of the PSD rats decreased (P < 0.05) compared with the normal control and stroke groups. On the contrary, the weights of the liquiritin and escitalopram groups increased and their sucrose consumption and activity increased in the open-field test (P < 0.05) compared with the PSD and normal saline (NS) groups.The result of immunofluorescence staining and western-blot showed that BDNF and Bcl-2 increased in the liquiritin group and Bax increased significantly in the stroke and PSD groups (P < 0.05).
Conclusions
Liquiritin is capable of inhibiting neuronal apoptosis in the hippocampus of PSD rats to improve depression symptoms. This improvement may be achieved by reducing the expression of Bax and increasing the expressions of Bcl-2 and BDNF in the hippocampus of PSD rats.