秘鲁亚马逊地区原生可可叶层中附生酵母的冻干和野外存活。

IF 1.4 4区 生物学
Eryka Gaslac-Zumaeta, Kelvin James Llanos-Gómez, Segundo Manuel Oliva-Cruz, Jorge Ronny Díaz-Valderrama
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引用次数: 0

摘要

附生酵母是一种很有前途的植物病害生物防治剂,但其保存和田间转移具有挑战性。在这里,我们研究了6种具有成本效益的冻干保护剂,以保存从秘鲁本地可可(可可可可)层球分离的7株亚马逊酵母菌。我们评估了酵母在离体冻干后30天和90天的生存能力,以及它们在田间控制接种可可果实后的生存能力。最佳保护剂为麦芽糖糊精、蜂蜜+脱脂牛奶和蜂蜜。用麦芽糖糊精冻干30 d后,异常Wickerhamomyces anomalus KLG-014和Wickerhamomyces sp. EGZ-38的存活率均高于97.3%。此外,假丝酵母sp. KLG-103与蜂蜜+脱脂牛奶冻干后30 d的活力大于50%。在90 d时,用蜂蜜+脱脂牛奶冻干后,异常W. KLG-014、可可汉娜菌KLG-063和赫氏Kwoniella heveanensis EGZ-07的存活率均大于20%,后者的存活率为100%。相反,海藻酸钠是最低的保护剂,因为酵母的活力为0%。在田间试验中,除海藻酸钠处理外,其余处理均能在30 d后从可可果实表面成功分离出W. anomalus KLG-014、K. heveanensis EGZ-07、Debaryomyces hansenii EGZ-31和Wickerhamomyces sp. EGZ-38。形态学和分子学证据证实了这一点。本研究表明,麦芽糖糊精、蜂蜜和脱脂牛奶适合于保证生物防治酵母在冻干后90天的体外生存能力,以及在田间接种可可果实后30天的生存能力。这是开发生物防治替代方案的第一步,利用来自秘鲁亚马逊的原生微生物来减轻可可病原体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lyophilization and Field Survival of Epiphytic Yeasts from the Phyllosphere of Native Cacao in the Peruvian Amazon.

Epiphytic yeasts are promising biocontrol agents of plant diseases but preserving and transferring them to the field is challenging. Here, we studied six cost-effective lyophilization protective agents to preserve seven strains of Amazonian yeast species isolated from the phyllosphere of native cacao (Theobroma cacao) in Peru. We evaluated the viability of yeasts at 30 and 90 days post-lyophilization in vitro, and their survival after controlled inoculation on cacao fruits in the field. The best protective agents were maltodextrin, honey + skim milk, and honey. Wickerhamomyces anomalus KLG-014 and Wickerhamomyces sp. EGZ-38 showed higher than 97.3% viability after 30 days when lyophilized with maltodextrin. Additionally, Candida sp. KLG-103 showed a viability greater than 50% after 30 days when lyophilized with honey + skim milk. At 90 days, W. anomalus KLG-014, Hannaella theobromatis KLG-063, and Kwoniella heveanensis EGZ-07 showed a viability greater than 20%, with the latter showing an outstanding 100% viability, when lyophilized with honey + skim milk. Conversely, sodium alginate was the least protective agent, as yeast showed 0% viability. In the field, W. anomalus KLG-014, K. heveanensis EGZ-07, Debaryomyces hansenii EGZ-31, and Wickerhamomyces sp. EGZ-38 were successfully re-isolated from the surface of cacao fruits under all treatments after 30 days, except for sodium alginate. This was corroborated via morphological and molecular evidence. This study demonstrates that maltodextrin, honey, and skim milk are suitable for ensuring the in vitro viability of biocontrol yeasts up to 90 days after lyophilization, and their survival up to 30 days after inoculation on cacao fruits in the field. This is a first step toward the development of a biocontrol alternative to mitigate cacao pathogens using native microorganisms from the Amazon in Peru.

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来源期刊
Biopreservation and Biobanking
Biopreservation and Biobanking Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology
自引率
12.50%
发文量
114
期刊介绍: Biopreservation and Biobanking is the first journal to provide a unifying forum for the peer-reviewed communication of recent advances in the emerging and evolving field of biospecimen procurement, processing, preservation and banking, distribution, and use. The Journal publishes a range of original articles focusing on current challenges and problems in biopreservation, and advances in methods to address these issues related to the processing of macromolecules, cells, and tissues for research. In a new section dedicated to Emerging Markets and Technologies, the Journal highlights the emergence of new markets and technologies that are either adopting or disrupting the biobank framework as they imprint on society. The solutions presented here are anticipated to help drive innovation within the biobank community. Biopreservation and Biobanking also explores the ethical, legal, and societal considerations surrounding biobanking and biorepository operation. Ideas and practical solutions relevant to improved quality, efficiency, and sustainability of repositories, and relating to their management, operation and oversight are discussed as well.
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