甲状旁腺激素促进纤维软骨干细胞软骨形成,缓解颞下颌关节骨性关节炎。

IF 4.4 4区 医学 Q2 CELL & TISSUE ENGINEERING
Zhihang Yue, Wuyi Gong, Haojun Chu, Yongming Li
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引用次数: 0

摘要

背景:甲状旁腺激素(PTH)可促进软骨下骨形成,缓解颞下颌关节(TMJ)骨关节炎(OA),但PTH对软骨表面纤维软骨干细胞(FCSCs)的影响尚不清楚。方法:建立大鼠TMJOA模型,给予甲状旁腺素治疗。采用显微计算机断层扫描、组织学和免疫组织化学染色对大鼠髁进行分析。为了研究PTH对体外FCSCs的影响,我们采用了定量聚合酶链反应、Western Blot和免疫荧光染色。在tdTomato中构建TMJOA模型;组织蛋白酶K (Ctsk)-Cre小鼠,用甲状旁腺激素拯救小鼠。采用EdU和免疫荧光染色法检测fscs在体内的增殖和软骨分化情况。此外,在椎间盘切除术后,我们向Ctsk-Cre注射白喉毒素(DT);白喉毒素受体(DTR)小鼠消融FCSCs。注射甲状旁腺素,免疫荧光染色评价COL2A1阳性区。结果:成功建立TMJOA模型,经甲状旁腺激素治疗后,大鼠髁突BV/TV和Tb均明显减少。Th升高,软骨相关基因表达升高。此外,PTH还能促进体外培养的FCSCs成软骨分化。在tdTomato;PTH给药后,Ctsk- cre小鼠Ctsk/EdU和Ctsk/COL2A1双阳性细胞增多。此外,在DT消融FCSCs后,PTH治疗的效果明显降低。结论:甲状旁腺素促进髁突FCSCs增殖和软骨分化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
PTH Promotes Chondrogenesis of Fibrocartilage Stem Cells and Alleviates Temporomandibular Joint Osteoarthritis.

Background: Parathyroid hormone (PTH) can promote subchondral bone formation and alleviate temporomandibular joint (TMJ) osteoarthritis (OA), but the effects of PTH on fibrocartilage stem cells (FCSCs) in cartilage surfaces have yet to be studied.

Methods: We established the TMJOA model in rats and administered PTH to treat them. Rat condyles were analyzed using micro-computed tomography, histological, and immunohistochemical staining. To study PTH's effects on FCSCs in vitro, we employed quantitative polymerase chain reaction, Western Blot, and immunofluorescence staining. We also constructed the TMJOA model in tdTomato; Cathepsin K (Ctsk)-Cre mice and rescued them with PTH. EdU and immunofluorescence staining were used to measure the proliferation and chondrogenic differentiation of FCSCs in vivo. Furthermore, after discectomy, we injected diphtheria toxin (DT) into the Ctsk-Cre; diphtheria toxin receptor (DTR) mice to ablate FCSCs. Afterwards, PTH was injected, and we evaluated the Collagen Type II Alpha 1 (COL2A1)-positive area using immunofluorescence staining.

Results: We successfully developed a TMJOA model, and after treatment with PTH, the rat condyles' BV/TV and Tb. Th increased, and the expression of chondrogenic-related genes was elevated. Additionally, PTH promoted the chondrogenic differentiation of FCSCs in vitro. In tdTomato; Ctsk-Cre mice, the Ctsk/EdU and Ctsk/COL2A1 double-positive cells were increased after PTH administration. Moreover, after the ablation of FCSCs by DT, the effects of PTH treatment were notably reduced.

Conclusion: PTH promotes the proliferation and chondrogenic differentiation of condylar FCSCs.

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来源期刊
Tissue engineering and regenerative medicine
Tissue engineering and regenerative medicine CELL & TISSUE ENGINEERING-ENGINEERING, BIOMEDICAL
CiteScore
6.80
自引率
5.60%
发文量
83
审稿时长
6-12 weeks
期刊介绍: Tissue Engineering and Regenerative Medicine (Tissue Eng Regen Med, TERM), the official journal of the Korean Tissue Engineering and Regenerative Medicine Society, is a publication dedicated to providing research- based solutions to issues related to human diseases. This journal publishes articles that report substantial information and original findings on tissue engineering, medical biomaterials, cells therapy, stem cell biology and regenerative medicine.
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