茄汁通过降低醋酸铅暴露Wistar大鼠肌酸酐激酶心肌带和atp酶活性发挥心脏保护作用

Journal of trace elements and minerals Pub Date : 2025-06-10 DOI:10.1016/j.jtemin.2025.100253

Olorunsola Israel Adeyomoye , Olugbemi Tope Olaniyan , Juliana Bunmi Adetunji , Oluwaseun Charles Adetunji

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引用次数: 0

摘要

铅暴露是已知的心血管疾病的危险因素，但有效的心脏保护干预措施仍未充分探索。尽管已有研究，但关于减轻铅引起的心脏损伤的潜在自然疗法的清晰度有限。目的研究茄茄乙酸乙酯提取物对铅暴露Wistar大鼠的心脏保护作用，探讨其治疗潜力和相关性。方法对新鲜番茄果实进行风干、研磨、浓缩、乙酸乙酯萃取。部分EESL用GC-MS分析。15只雄性Wistar大鼠随机分为3组（每组5只）：对照组（蒸馏水）、醋酸铅（0.5 mg.mL-1）和醋酸铅+ EESL （5 mg.kg-1）。醋酸铅和EESL分别通过饮水和灌胃给予4周。分别用称重秤和火焰原子吸收光谱法测定体重和铅浓度。采用分光光度法测定抗氧化剂和心脏生物标志物。数据分析采用方差分析，显著性水平为p <；0.05.结果鉴定出56种成分，其中6-脱氢孕酮（-9.3 kcal/mol）对β -肾上腺素能受体的对接亲和力最高。与醋酸铅组相比，醋酸铅+ EESL组的体重显著增加。与醋酸铅+ EESL组相比，醋酸铅组的铅浓度显著升高。与醋酸铅组相比，乙酸铅+ EESL组SOD活性显著升高，CK-MB、Na + /K + ATPase、ca2 + ATPase活性显著降低。结论茄叶乙酸乙酯提取物可提高铅暴露大鼠的抗氧化活性，降低心脏损伤标志物，具有保护心脏的作用。然而，相关的体重增加引起了对潜在代谢风险的担忧，需要进一步研究

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Solanum lycopersicum exerts cardioprotective effects via reduced creatinine kinase myocardial band and ATPase activities in Wistar rats exposed to lead acetate

Background

Lead exposure is a known risk factor for cardiovascular diseases, yet effective cardioprotective interventions remain inadequately explored. Despite existing research, there is limited clarity on potential natural therapies that can mitigate lead-induced cardiac damage.

Objective

This study investigates the cardioprotective effects of ethyl acetate extract of Solanum lycopersicum (EESL) in lead-exposed Wistar rats, providing insights into its therapeutic potential and relevance.

Methods

Fresh fruits of Solanum lycopersicum (tomatoes) were air-dried, ground, concentrated, and extracted using ethyl acetate. A portion of EESL was analyzed using GC–MS. Fifteen (15) male Wistar rats were randomly assigned to three groups (n = 5 per group): control (distilled water), lead acetate (0.5 mg.mL^-1), and lead acetate + EESL (5 mg.kg^-1). Lead acetate and EESL were administered via drinking water and oral gavage, respectively, for four weeks. Body weight and lead concentration were measured using a weighing scale and flame atomic absorption spectrometry, respectively. Antioxidants and cardiac biomarkers were assessed using spectrophotometry. Data were analyzed using ANOVA at a significance level of p < 0.05.

Results

Gas chromatography mass spectrometry identified 56 constituents, with 6-dehydroprogesterone (-9.3 kcal/mol) showing the highest docking affinity for the beta-adrenergic receptor. There was a significant increase in body weight in the lead acetate + EESL group compared to the lead acetate group. Lead concentration was significantly higher in the lead acetate group compared to the lead acetate + EESL group. SOD activity significantly increased in the lead acetate + EESL group, while CK-MB, Na⁺/K⁺ ATPase, and Ca²⁺ ATPase activities significantly decreased compared to the lead acetate group.

Conclusion

Solanum lycopersicum ethyl acetate extract demonstrated cardioprotective effects by enhancing antioxidant activity and reducing cardiac injury markers in lead-exposed rats. However, the associated increase in body weight raises concerns about potential metabolic risks, warranting further investigation

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来源期刊

Journal of trace elements and minerals Medicine and Dentistry (General), Analytical Chemistry, Environmental Science (General), Toxicology, Biochemistry, Genetics and Molecular Biology (General), Nutrition, Veterinary Science and Veterinary Medicine (General)

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审稿时长

65 days