野火衍生的热原溶解有机物(pyDOM)增强河流DOM反应性和氮代谢

IF 11.3 1区 环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL
Mingxing Cao, Hua Ma*, Yixuan Ye, Sheng-Ao Li, Xinghong Cao, Hai Huang, Zhe Li and Fuyi Cui, 
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引用次数: 0

摘要

野火深刻地重塑了土壤有机质组成,并对全球碳循环产生级联影响,然而,热成因溶解有机质(pyDOM)对河流DOM反应性和微生物代谢的生物地球化学影响仍不清楚。在这里,我们对从受野火影响的土壤和未受野火影响的土壤中提取的DOM进行了对照培养,以评估DOM分子转化和微生物反应。高分辨率质谱分析和底物显式建模表明,pyDOM引入了具有高修饰芳香指数(AImod)和双键当量(DBE)的难熔成分(如缩合芳烃和单宁)。反应组学分析表明,pyDOM表现出增强的反应活性,这可能与大分子电子穿梭和水溶性的改变有关。虽然pyDOM的引入降低了河流微生物的总体多样性和丰度,但它使丝状蓝藻的丰度增加了17倍,同时提高了河流微生物的自养能力和与氮代谢相关的功能丰度。PICRUSt2分析的基因组证据表明,pydom驱动的反硝化途径富集,特别是通过上调质周硝酸盐还原酶组分(napA + 3.0倍;napB + 3.1倍),提示好氧反硝化能力增强。这些发现表明,pyDOM是一种生物地球化学载体,可以将陆地碳封存重新定向到水生代谢网络中,强调需要将pyDOM通量纳入与气候相关的生物地球化学框架。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Wildfire-Derived Pyrogenic Dissolved Organic Matter (pyDOM) Enhances Riverine DOM Reactivities and Nitrogen Metabolisms

Wildfire-Derived Pyrogenic Dissolved Organic Matter (pyDOM) Enhances Riverine DOM Reactivities and Nitrogen Metabolisms

Wildfires profoundly reshape soil organic matter composition with cascading impacts on global carbon cycling, yet, the biogeochemical consequences of pyrogenic dissolved organic matter (pyDOM) on riverine DOM reactivity and microbial metabolism remain poorly constrained. Here, we conducted controlled incubation of river water amended with DOM extracted from wildfire-affected versus undisturbed soils to assess molecular DOM transformations and microbial responses. High-resolution mass spectrometry and substrate-explicit modeling revealed that pyDOM introduction increased refractory components (e.g., condensed aromatics and tannins) with high modified aromaticity index (AImod) and double bond equivalents (DBE). Reactomics analysis revealed that pyDOM exhibited enhanced reactivity which may be associated with alterations in macromolecular electron shuttles and water solubility. While pyDOM introduction reduced overall riverine microbial diversity and abundance, it triggered a 17-fold increase of filamentous cyanobacteria abundance, simultaneously boosting both autotrophic capabilities and the functional abundance related to nitrogen metabolism in riverine microorganisms. Genomic evidence from PICRUSt2 analysis demonstrated pyDOM-driven enrichment of denitrification pathways, particularly through upregulation of periplasmic nitrate reductase components (napA + 3.0-fold; napB + 3.1-fold), suggesting enhanced aerobic denitrification capacity. These findings establish pyDOM as a biogeochemical vector that redirects terrestrial carbon sequestration into aquatic metabolic network, emphasizing the need to integrate pyDOM fluxes into climate-relevant biogeochemical frameworks.

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来源期刊
环境科学与技术
环境科学与技术 环境科学-工程:环境
CiteScore
17.50
自引率
9.60%
发文量
12359
审稿时长
2.8 months
期刊介绍: Environmental Science & Technology (ES&T) is a co-sponsored academic and technical magazine by the Hubei Provincial Environmental Protection Bureau and the Hubei Provincial Academy of Environmental Sciences. Environmental Science & Technology (ES&T) holds the status of Chinese core journals, scientific papers source journals of China, Chinese Science Citation Database source journals, and Chinese Academic Journal Comprehensive Evaluation Database source journals. This publication focuses on the academic field of environmental protection, featuring articles related to environmental protection and technical advancements.
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