PEARL：投影估计加速Richardson-Lucy反褶积快速体成像。

IF 3.2 2区物理与天体物理 Q2 OPTICS

Optics express Pub Date : 2025-06-02 DOI:10.1364/OE.559124

Shunan Wu, Wenhao Liu, Xuanwen Hua, Jiajia Luo, Xunbin Wei, Shu Jia

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引用次数: 0

摘要

生物标本的三维成像需要高时空分辨率，但处理连续记录图像的大量计算需求阻碍了毫秒级细胞事件的实时重建。在这里，我们介绍了投影估计加速Richardson-Lucy （PEARL）反卷积，这是一种加速的3D重建技术，可以显着降低基于Richardson-Lucy反卷积的图像重建的迭代要求。我们通过成像免疫染色的线粒体、gfp标记的过氧化物酶体和人类多能干细胞衍生的类器官来验证PEARL的能力，证明了在不同的生物学应用中增强的重建速度和准确性。

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PEARL: projection-estimation accelerated Richardson-Lucy deconvolution for rapid volumetric imaging.

Three-dimensional imaging of biological specimens requires high spatiotemporal resolution, but the intensive computational demands of processing continuously recorded images hinder the real-time reconstruction of millisecond cellular events. Here, we introduce projection-estimation accelerated Richardson-Lucy (PEARL) Deconvolution, an accelerated 3D reconstruction technique that significantly reduces iteration requirements for image reconstruction based on Richardson-Lucy deconvolution. We validate PEARL's capabilities by imaging immuno-stained mitochondria, GFP-labeled peroxisomes, and human pluripotent stem cell-derived organoids, demonstrating enhanced reconstruction speed and accuracy across diverse biological applications.

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来源期刊

Optics express 物理-光学

CiteScore

6.60

自引率

15.80%

发文量

5182

审稿时长

2.1 months

期刊介绍： Optics Express is the all-electronic, open access journal for optics providing rapid publication for peer-reviewed articles that emphasize scientific and technology innovations in all aspects of optics and photonics.