基于分子印迹光子聚多巴胺的双信号传感器检测氧化应激生物标志物尿囊素

IF 5.5 3区材料科学 Q1 ELECTROCHEMISTRY

Electrochimica Acta Pub Date : 2025-06-14 DOI:10.1016/j.electacta.2025.146718

Akmaral Suleimenova , Ana C. Marques , Manuela F. Frasco , Elvira Fortunato , M. Goreti F. Sales

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引用次数: 0

摘要

提出了一种在分子印迹聚合物（MIP）传感器中分配两种传感器原理的仿生检测方法。该器件建立在透明的三电极导电氧化铟锡（ITO）系统上，该系统采用激光直接写入法在玻璃基板上制备。传感层是在尿囊素存在的情况下，在胶体二氧化硅颗粒上电聚合多巴胺制备的，胶体二氧化硅颗粒由于其短范围有序结构而呈现出结构色。这种光电-电化学双信号输出成功地用于检测氧化应激生物标志物尿囊素。通过电化学阻抗谱和结构色的反射率分析对其分析性能进行了评价。该传感器在合成尿中尿囊素浓度（0.1 nmol L−1至10000 nmol L−1）的宽范围内显示出线性响应。正如预期的那样，电化学信号在尿液中的最低检出限为0.012 nmol L−1。此外，作为干扰物的其他尿氧化应激代谢物尿酸和8-羟基-2′-脱氧鸟苷对尿囊素双信号检测无影响。材料的仿生和成本效益特性，结合改进的光电电化学检测的分析特性，为尿液分析中氧化生物标志物的筛选提供了一个具有巨大潜力的传感器平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A dual-signal sensor based on molecularly imprinted photonic polydopamine for detection of the oxidative stress biomarker allantoin

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A dual-signal sensor based on molecularly imprinted photonic polydopamine for detection of the oxidative stress biomarker allantoin

A novel biomimetic detection method is presented allocating two transducer principles in a molecularly imprinted polymer (MIP)-based sensor. The device was constructed on a transparent three-electrode system of conductive indium tin oxide (ITO) fabricated by laser direct writing on glass substrates. The sensing layer was prepared by electropolymerizing dopamine in the presence of allantoin, on colloidal silica particles that exhibited a structural color due to the short-range ordered structure. This opto-electrochemical dual-signal output was successfully developed for the detection of the oxidative stress biomarker allantoin. The analytical properties were evaluated by electrochemical impedance spectroscopy and reflectance analysis of the structural color. The sensor showed a linear response over a wide range of allantoin concentrations (0.1 nmol L⁻¹ to 10000 nmol L⁻¹) measured in synthetic urine. As expected, the lowest limit of detection in urine (0.012 nmol L⁻¹) was achieved with the electrochemical signal. In addition, other urinary oxidative stress metabolites tested as interferents, namely uric acid and 8-hydroxy-2′-deoxyguanosine, had no effect on the dual-signal detection of allantoin. The biomimetic and cost-effective properties of the materials in combination with the improved analytical properties of opto-electrochemical detection provide a sensor platform with great potential for the screening of oxidative stress biomarkers in urinalysis.

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来源期刊

Electrochimica Acta 工程技术-电化学

CiteScore

11.30

自引率

6.10%

发文量

1634

审稿时长

41 days

期刊介绍： Electrochimica Acta is an international journal. It is intended for the publication of both original work and reviews in the field of electrochemistry. Electrochemistry should be interpreted to mean any of the research fields covered by the Divisions of the International Society of Electrochemistry listed below, as well as emerging scientific domains covered by ISE New Topics Committee.