危重犬血清透明质酸及静脉输液治疗的影响

IF 2.6 3区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
PLoS ONE Pub Date : 2025-06-13 eCollection Date: 2025-01-01 DOI:10.1371/journal.pone.0325809
Manon Rigot, Alexa M Bersenas, Shane W Bateman, Shauna L Blois, Gabrielle Monteith, R Darren Wood
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引用次数: 0

摘要

背景:内皮糖萼(EG)似乎在生理血管内皮功能中起关键作用。败血症、创伤和失血性休克与EG脱落有关,静脉输液有可能使EG降解恶化。很少有可用的研究评估静脉输液、炎症和危重犬EG降解之间的关系。目的:研究危重犬住院后48小时内EG的降解,并描述静脉输液和炎症对EG的影响。方法:在48小时内的5个预先设定的时间点测量非肺脓毒症、肺脓毒症或自发性腹膜出血犬的透明质酸(HA),这是EG降解的生物标志物。血凝素浓度随时间变化趋势,在组间进行比较,并研究其与静脉输液累积量、促炎细胞因子(白细胞介素-6,IL-6)和高血容量生物标志物(房钠肽,ANP)的关系。结果:各组间各时间点HA浓度差异无统计学意义。在脓毒症和自发性腹膜出血患者中,它在研究的前24小时增加,然后达到平台期。各组患者IL-6浓度与HA浓度呈显著正相关(p = 0.026)。各组患者住院期间累积液量与血凝素浓度有显著相关性(p = 0.0002)。ANP对HA浓度无显著影响。血凝素浓度与疾病严重程度相关,但与预后无关。结论:在所研究的狗中,炎症标记物和大量静脉输液与血凝素浓度增加有关,因此推测EG降解。静脉输液治疗对脑电图的临床影响有待进一步研究。临床医生在为危重犬开液体复苏处方时应仔细考虑这些发现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Serum hyaluronic acid in critically ill dogs and influence of intravenous fluid therapy.

Serum hyaluronic acid in critically ill dogs and influence of intravenous fluid therapy.

Serum hyaluronic acid in critically ill dogs and influence of intravenous fluid therapy.

Serum hyaluronic acid in critically ill dogs and influence of intravenous fluid therapy.

Background: The endothelial glycocalyx (EG) appears to play a critical role in physiological vasculo-endothelial function. Sepsis, trauma, and hemorrhagic shock are associated with EG shedding and intravenous fluids have the potential to worsen EG degradation. There is little available research evaluating the relationship between intravenous fluids, inflammation, and EG degradation in critically ill dogs.

Objective: To study EG degradation in critically ill dogs over their first 48 hours of hospitalization and characterize the influence of intravenous fluids and inflammation.

Methods: Hyaluronic acid (HA), a biomarker of EG degradation, was measured in dogs with non-pulmonary sepsis, pulmonary sepsis, or spontaneous hemoperitoneum at five pre-defined time points over 48 hours. The concentration of HA was trended over time, compared between groups, and studied for associations with the cumulative volume of intravenous fluids administered, a pro-inflammatory cytokine (interleukin-6, IL-6), and a biomarker of hypervolemia (atrial natriuretic peptide, ANP).

Results: Concentration of HA was not significantly different between the groups at each time point. It increased over the first 24 hours of the study before reaching a plateau in patients with sepsis and spontaneous hemoperitoneum. Concentration of IL-6 had a significant positive association with HA concentration on presentation in all groups (p = 0.026). Cumulative fluid volume had a significant association with HA concentration during hospitalization in all groups (p = 0.0002). There was no significant effect of ANP on HA concentration. Concentration of HA was associated with disease severity but not with outcome.

Conclusions: In the dogs studied, markers of inflammation and administration of larger volumes of intravenous fluids were associated with increasing HA concentration, and thus presumptive EG degradation. Further research is needed to explore the clinical impact of intravenous fluid therapy on the EG. These findings should be considered carefully by clinicians prescribing fluid resuscitation for critically ill dogs.

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来源期刊
PLoS ONE
PLoS ONE 生物-生物学
CiteScore
6.20
自引率
5.40%
发文量
14242
审稿时长
3.7 months
期刊介绍: PLOS ONE is an international, peer-reviewed, open-access, online publication. PLOS ONE welcomes reports on primary research from any scientific discipline. It provides: * Open-access—freely accessible online, authors retain copyright * Fast publication times * Peer review by expert, practicing researchers * Post-publication tools to indicate quality and impact * Community-based dialogue on articles * Worldwide media coverage
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