Assessment of Retention and Penetration Depth of Different Pit and Fissure Sealants: An In-vitro Study.

Aim: This in-vitro study aims to access the extent of penetration and retention of different sealants in deciduous molars.

Materials and methods: Thirty extracted deciduous molars were split into three groups according to the kind of sealants applied. The specimens were divided into the equal numbers after acid etching and sealant application in Group 1: Conventional sealant (Fuji VII GIC, GC Corporation, Tokyo, Japan), Group 2: Colored resin-based-fluoride fissure sealant (Delton FS+ Dentsply, Germany), and Group 3: Nano-silver-reinforced fissure sealant (Kids-e-dental LLP, India) was done. Prepared samples were thermocycle and examined for retention using Simonsen's criterion. Further these samples were coated with nail varnish and soaked in methylene blue solution (1%) for a day and cleaned with distilled water. Buccolingual sectioning of prepared samples was done to examined under a stereomicroscope (SEM) at a magnification of 10X and score by trained examiner based on the sealant penetration depth criteria MLC Fracasso in millimeters. The Non-Parametric Kruskal Wallis H test was used to analyses all the data. 100% retention was found in group 3 followed by group 2 then 1.

Result and conclusion: Frequency sealant penetration depth comparison was found significant difference between the groups. Nano-silver-reinforced sealant represented improved retention and penetration as related to the resin-based-fluoride fissure sealant and conventional sealants.