表面修饰的99mtc -乳铁蛋白纳米颗粒作为前哨淋巴结定位的示踪剂。

IF 4.6 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2025-06-10 DOI:10.1016/j.nano.2025.102835

Sanjay Kulkarni PhD , Anuj Kumar MSc , Soji Soman PhD , Abhijeet Pandey PhD , Suresh Subramanian PhD , Srinivas Mutalik PhD

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引用次数: 0

摘要

我们之前研究了放射性锝-99米（99mTc）标记的乳铁蛋白纳米颗粒（LF-NPs）用于前哨淋巴结（SLN）定位的使用。与基于人血清白蛋白的商业示踪剂相比，LF-NPs具有更好的尺寸均匀性和减少批次差异。然而，尽管99mTc-LF-NPs在RAW 264.7巨噬细胞中的体外试验很有希望，但体内结果显示其在sln中的积累较差。为了提高它们的体内性能，我们用不同的试剂对LF-NPs进行了表面改性，包括聚乙二醇（PEG）。表征研究表明，聚乙二醇化的LF-NPs （LF-NP@PEG）是最有效的。体外试验显示，与未修饰的LF-NPs相比，LF-NP@PEG的细胞摄取显著增加。动物模型的体内评估显示，SLN的摄取和保留增加，这得到了科学成像研究的支持。虽然需要进一步的改进，但这些发现表明了修饰的LF-NPs在SLN检测中的潜在应用。

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Surface-modified 99mTc-Lactoferrin nanoparticles as tracers for sentinel lymph node mapping

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Surface-modified 99mTc-Lactoferrin nanoparticles as tracers for sentinel lymph node mapping

We had previously examined the use of radioactive technetium-99m (Tc-99m)-labelled lactoferrin nanoparticles (LF-NPs) for sentinel lymph node (SLN) mapping. Compared with commercial tracer based on human serum albumin, LF-NPs exhibited better size uniformity and reduced batch variation. However, while in vitro tests with ^99mTc-LF-NPs in RAW 264.7 macrophages were promising, the in vivo results revealed poor accumulation in the SLNs. To improve their in vivo performance, we performed surface modification of LF-NPs with different agents, including polyethylene glycol (PEG). Characterisation studies revealed that PEGylated LF-NPs (LF-NP@PEG) were the most effective. In vitro tests revealed significantly greater cellular uptake of LF-NP@PEG than unmodified LF-NPs. In vivo evaluation in animal model demonstrated increased SLN uptake and retention, which was supported by scintigraphic imaging studies. Although further refinements are needed, these findings suggest potential application for modified LF-NPs in SLN detection.

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来源期刊

Nanomedicine : nanotechnology, biology, and medicine 工程技术-纳米科技

CiteScore

11.10

自引率

0.00%

发文量

133

审稿时长

42 days

期刊介绍： The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.