固定化不动单胞菌（Opacimonas immobilis sp. nov.），一种新的无鞭毛的Alteromonadaceae物种，其基因组最小，分离自硅藻Actinocyclus curvatulus藻球。

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2025-06-13 DOI:10.1007/s00203-025-04375-3

Xiaoyu Yang, Xuanru Lin, Runlin Cai, Hui Wang, Guanjing Cai

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引用次数: 0

摘要

从弯曲放线藻藻球中分离到一株革兰氏染色阴性、椭圆形、需氧、无运动的菌株LMIT016T。基于16S rRNA基因序列构建的系统发育树和GTDB-Tk系统发育分析结果表明，菌株LMIT016T与O. viscosa LMIT007T亲缘关系较近（16S rRNA基因序列同源率为96.08%）。菌株LMIT016T与科内相关型种的平均核苷酸一致性值在76.37 ~ 79.96%之间，数字DNA-DNA杂交值在30.0%以下，证实了它们代表不同的种。值得注意的是，菌株LMIT016T在Alteromonadaceae家族中拥有最小的基因组（2.53 Mbp），完全缺乏鞭毛组装和趋化性相关基因。然而，比较基因组分析显示，它保持了对藻圈生活方式的适应功能，如生物膜形成、碳水化合物代谢、维生素和类胡萝卜素合成。菌株LMIT016T在1.0 ~ 5.0% NaCl、pH 6 ~ 10、温度20 ~ 35℃条件下生长最佳。主要的呼吸醌是泛醌-8。主要脂肪酸为C16:1ω7c/C16:1ω6c（32.4%）、C16:0（17.3%）和C18:1ω7c/C18:1ω6c(31.0%)，主要极性脂质为磷脂酰乙醇胺、氨基磷脂和磷脂酰甘油。这些发现，结合系统发育和基因型特征，证实菌株LMIT016T为Opacimonas immobilis sp. nov.的新种，其类型菌株LMIT016T （= KCTC 92596 T = MCCC 1K08254T）。该菌株的鉴定丰富了对Opacimonas属微生物多样性的理解，并突出了栖息于藻圈的特殊细菌进化。

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Opacimonas immobilis sp. nov., a novel non-flagellated Alteromonadaceae species with the smallest genome isolated from the phycosphere of the diatom Actinocyclus curvatulus.

A bacterial strain LMIT016^T, characterized as Gram-stain-negative, oval shaped, aerobic and non-motile, was isolated from the phycosphere of the diatom Actinocyclus curvatulus. Both the phylogenetic tree constructed from 16S rRNA gene sequences and the phylogenomic analysis using GTDB-Tk revealed that strain LMIT016^T was closely related to O. viscosa LMIT007^T (96.08% of 16S rRNA gene sequence identity). Strain LMIT016^T and related type species in the family exhibited average nucleotide identity values ranging from 76.37 to 79.96%, with digital DNA-DNA hybridization values below 30.0%, confirming that they represent different species. Notably, strain LMIT016^T possesses the smallest genome (2.53 Mbp) among Alteromonadaceae family, completely lacking genes related to flagellar assembly and chemotaxis. However, comparative genomic analysis revealed that it kept adaptive functions for phycosphere lifestyle, such as biofilm formation, carbohydrate metabolism, and vitamin and carotenoid synthesis. Further, strain LMIT016^T exhibited optimal growth under conditions of 1.0-5.0% NaCl, pH 6-10, and temperatures between 20 and 35 ℃. The major respiratory quinone was ubiquinone-8. The major fatty acids (> 10%) were identified as C_16:1ω7c/C_16:1ω6c (32.4%), C_16:0 (17.3%) and C_18:1ω7c/C_18:1ω6c (31.0%), while the major polar lipids comprised phosphatidylethanolamine, aminophospholipid and phosphatidylglycerol. These findings, combined with the phylogenetic and genotypic features, confirmed that strain LMIT016^T represented a novel species in the genus Opacimonas, for which the name Opacimonas immobilis sp. nov. was proposed, with type strain LMIT016^T (= KCTC 92596^T = MCCC 1K08254^T). The identification of this strain enriches the understanding of microbial diversity within the genus Opacimonas and highlights the specialized bacterial evolution for inhabiting the phycosphere.

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.