Ahmed Fotouh, Nady Khairy Elbarbary, Maha Abdullah Momenah, Mohsen A Khormi, Wafaa H Mohamed, Hoda S Sherkawy, Ahmed Ezzat Ahmed, Mohamed Diab, Said Elshafae
{"title":"间充质干细胞对四氯化碳诱导大鼠肝毒性的保护作用：肝脏参数的恢复和组织病理学评价。","authors":"Ahmed Fotouh, Nady Khairy Elbarbary, Maha Abdullah Momenah, Mohsen A Khormi, Wafaa H Mohamed, Hoda S Sherkawy, Ahmed Ezzat Ahmed, Mohamed Diab, Said Elshafae","doi":"10.2460/ajvr.25.03.0074","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the therapeutic potential of mesenchymal stem cells (MSCs) in alleviating carbon tetrachloride (CCl4)-induced liver damage in rats.</p><p><strong>Methods: </strong>The study was conducted from October 2024 through February 2025. A total of 80 rats (20 rats for MSC isolation and 60 additional rats) were divided into groups. The negative control groups received olive oil twice weekly for 9 weeks, a positive control group received CCl4 in olive oil twice weekly for 9 weeks, and an MSC test group received the same treatment as the positive control group, followed by treatment with MSCs once a week for 4 weeks. Liver enzyme levels (ALT, ASP, and ALP) and the oxidant-antioxidant parameters (glutathione peroxidase, superoxide dismutase, malondialdehyde, glutathione peroxidase, catalase activity, and total antioxidant capacity) were assessed. Histopathological analysis of liver tissues was performed to examine the extent of liver damage.</p><p><strong>Results: </strong>The results showed increases in liver enzymes and oxidative stress markers in the positive control group compared to the negative control group. The MSC test group exhibited reduced liver enzyme levels, enhanced antioxidant activity, and improved histological findings, with reduced inflammation and fibrosis.</p><p><strong>Conclusions: </strong>MSCs possess a potent hepatoprotective effect through the restoration of antioxidant defenses, reduction of oxidative stress, and promotion of tissue regeneration.</p><p><strong>Clinical relevance: </strong>This study supports the potential of MSC-based therapy as a promising approach for the treatment of liver damage caused by toxic insults, such as CCl4.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-10"},"PeriodicalIF":1.3000,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Hepatoprotective effects of mesenchymal stem cells in carbon tetrachloride-induced liver toxicity in rats: restoration of liver parameters and histopathological evaluation.\",\"authors\":\"Ahmed Fotouh, Nady Khairy Elbarbary, Maha Abdullah Momenah, Mohsen A Khormi, Wafaa H Mohamed, Hoda S Sherkawy, Ahmed Ezzat Ahmed, Mohamed Diab, Said Elshafae\",\"doi\":\"10.2460/ajvr.25.03.0074\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To evaluate the therapeutic potential of mesenchymal stem cells (MSCs) in alleviating carbon tetrachloride (CCl4)-induced liver damage in rats.</p><p><strong>Methods: </strong>The study was conducted from October 2024 through February 2025. A total of 80 rats (20 rats for MSC isolation and 60 additional rats) were divided into groups. The negative control groups received olive oil twice weekly for 9 weeks, a positive control group received CCl4 in olive oil twice weekly for 9 weeks, and an MSC test group received the same treatment as the positive control group, followed by treatment with MSCs once a week for 4 weeks. Liver enzyme levels (ALT, ASP, and ALP) and the oxidant-antioxidant parameters (glutathione peroxidase, superoxide dismutase, malondialdehyde, glutathione peroxidase, catalase activity, and total antioxidant capacity) were assessed. Histopathological analysis of liver tissues was performed to examine the extent of liver damage.</p><p><strong>Results: </strong>The results showed increases in liver enzymes and oxidative stress markers in the positive control group compared to the negative control group. The MSC test group exhibited reduced liver enzyme levels, enhanced antioxidant activity, and improved histological findings, with reduced inflammation and fibrosis.</p><p><strong>Conclusions: </strong>MSCs possess a potent hepatoprotective effect through the restoration of antioxidant defenses, reduction of oxidative stress, and promotion of tissue regeneration.</p><p><strong>Clinical relevance: </strong>This study supports the potential of MSC-based therapy as a promising approach for the treatment of liver damage caused by toxic insults, such as CCl4.</p>\",\"PeriodicalId\":7754,\"journal\":{\"name\":\"American journal of veterinary research\",\"volume\":\" \",\"pages\":\"1-10\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-06-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"American journal of veterinary research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2460/ajvr.25.03.0074\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2460/ajvr.25.03.0074","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Hepatoprotective effects of mesenchymal stem cells in carbon tetrachloride-induced liver toxicity in rats: restoration of liver parameters and histopathological evaluation.
Objective: To evaluate the therapeutic potential of mesenchymal stem cells (MSCs) in alleviating carbon tetrachloride (CCl4)-induced liver damage in rats.
Methods: The study was conducted from October 2024 through February 2025. A total of 80 rats (20 rats for MSC isolation and 60 additional rats) were divided into groups. The negative control groups received olive oil twice weekly for 9 weeks, a positive control group received CCl4 in olive oil twice weekly for 9 weeks, and an MSC test group received the same treatment as the positive control group, followed by treatment with MSCs once a week for 4 weeks. Liver enzyme levels (ALT, ASP, and ALP) and the oxidant-antioxidant parameters (glutathione peroxidase, superoxide dismutase, malondialdehyde, glutathione peroxidase, catalase activity, and total antioxidant capacity) were assessed. Histopathological analysis of liver tissues was performed to examine the extent of liver damage.
Results: The results showed increases in liver enzymes and oxidative stress markers in the positive control group compared to the negative control group. The MSC test group exhibited reduced liver enzyme levels, enhanced antioxidant activity, and improved histological findings, with reduced inflammation and fibrosis.
Conclusions: MSCs possess a potent hepatoprotective effect through the restoration of antioxidant defenses, reduction of oxidative stress, and promotion of tissue regeneration.
Clinical relevance: This study supports the potential of MSC-based therapy as a promising approach for the treatment of liver damage caused by toxic insults, such as CCl4.
期刊介绍：
The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.
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