咖喱叶种子(Murraya koenigii L.)衍生碳点作为光致发光探针用于HCT 116癌细胞中Fe3+离子的纳米摩尔检测和生物成像

IF 2 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Diraviam Jeeva, Sonaimuthu Mohandoss, Kuppu Sakthi Velu, Naushad Ahmad, Mohammad Aslam, Govindaraju Bhuvanalogini, Seong-Cheol Kim
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引用次数: 0

摘要

由天然产物合成的碳点由于其独特的性能而备受关注。本研究以咖喱叶种子(Murraya koenigii L.)为原料,在不使用任何表面钝化剂和处理的情况下,在140°C的水热条件下合成了光致发光碳点(CDs)。采用紫外可见光谱(UV-visible)、光致发光光谱(PL)、傅里叶变换红外光谱(FT-IR)、x射线衍射(XRD)、高分辨率透射电子显微镜(hrtem)和x射线光电子能谱(XPS)对咖喱叶种子碳点(Cls-CDs)进行了进一步的表征。Cls-CDs的尺寸为3.89±0.34 nm,在溶液紫外光照射下,在330 nm处激发时,在455nm处发出蓝色发光,量子产率为6.4%。此外,Cls-CDs具有优异的PL活性、激发依赖性发射和高pH稳定性。更有趣的是,Fe3+被发现选择性地淬灭Cls-CDs的PL。随着Fe3+离子浓度的增加,CDs在455nm处的发射峰强度逐渐猝灭,因此可以在0-25µM范围内绘制校准曲线,检测限为78.0 nm。可逆性研究证实,在Cls-CDs中加入廉价且易于获得的EDTA配体可以恢复PL,从而具有出色的可重复使用性,可用于检测Fe3+离子。最后,利用MTT试验探索了Cls-CDs在HCT-116细胞系中的细胞成像,发现细胞存活率超过90%,表明其在生物成像应用中的巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Curry Leaf Seeds (Murraya koenigii L.)-Derived Carbon Dots as Photoluminescent Probe for Nanomolar Detection of Fe3+ Ions and Bioimaging in HCT 116 Cancer Cells

Curry Leaf Seeds (Murraya koenigii L.)-Derived Carbon Dots as Photoluminescent Probe for Nanomolar Detection of Fe3+ Ions and Bioimaging in HCT 116 Cancer Cells

Curry Leaf Seeds (Murraya koenigii L.)-Derived Carbon Dots as Photoluminescent Probe for Nanomolar Detection of Fe3+ Ions and Bioimaging in HCT 116 Cancer Cells

Curry Leaf Seeds (Murraya koenigii L.)-Derived Carbon Dots as Photoluminescent Probe for Nanomolar Detection of Fe3+ Ions and Bioimaging in HCT 116 Cancer Cells

Carbon dots (CDs) synthesized from natural products have drawn numerous attention due to some unique properties. In this work, we synthesized photoluminescent carbon dots (CDs) using Curry leaf seeds (Murraya koenigii L.) via hydrothermal method at 140 °C for 6 h without using any further treatment or surface passivating agents. The curry leaf seeds-based carbon dots (Cls-CDs) were synthesized further characterized by UV-visible, Photoluminescence (PL) spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), and X-ray photoelectron spectroscopy (XPS). The size of Cls-CDs was 3.89 ± 0.34 nm, and they exhibited blue-color emission at 455 nm upon excitation at 330 nm under UV illumination in solution with a quantum yield of 6.4%. In addition, Cls-CDs show excellent PL activity, excitation-dependent emission, and high pH stability. More interestingly, Fe3+ is found to selectively quenching the PL of Cls-CDs over the several metal ions. The emission peak intensity of CDs at 455 nm was gradually quenched with increasing concentration of Fe3+ ion, thereby Fe3+ ion was quantified by plotting calibration curve over the range of 0–25 µM with a detection limit of 78.0 nM. The studies on reversibility confirmed that incorporating an inexpensive and easily obtainable EDTA ligand into the Cls-CDs leads to the restoration of PL, resulting in outstanding reusability for detecting Fe3+ ions. Finally, the Cls-CDs are explored for cell imaging in HCT-116 cell lines using a MTT assay, revealing cell viabilities exceeding 90%, suggesting their great potential in bioimaging applications.

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来源期刊
ChemistrySelect
ChemistrySelect Chemistry-General Chemistry
CiteScore
3.30
自引率
4.80%
发文量
1809
审稿时长
1.6 months
期刊介绍: ChemistrySelect is the latest journal from ChemPubSoc Europe and Wiley-VCH. It offers researchers a quality society-owned journal in which to publish their work in all areas of chemistry. Manuscripts are evaluated by active researchers to ensure they add meaningfully to the scientific literature, and those accepted are processed quickly to ensure rapid online publication.
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