Diraviam Jeeva, Sonaimuthu Mohandoss, Kuppu Sakthi Velu, Naushad Ahmad, Mohammad Aslam, Govindaraju Bhuvanalogini, Seong-Cheol Kim
{"title":"咖喱叶种子(Murraya koenigii L.)衍生碳点作为光致发光探针用于HCT 116癌细胞中Fe3+离子的纳米摩尔检测和生物成像","authors":"Diraviam Jeeva, Sonaimuthu Mohandoss, Kuppu Sakthi Velu, Naushad Ahmad, Mohammad Aslam, Govindaraju Bhuvanalogini, Seong-Cheol Kim","doi":"10.1002/slct.202501909","DOIUrl":null,"url":null,"abstract":"<p>Carbon dots (CDs) synthesized from natural products have drawn numerous attention due to some unique properties. In this work, we synthesized photoluminescent carbon dots (CDs) using Curry leaf seeds (<i>Murraya koenigii</i> L.) via hydrothermal method at 140 °C for 6 h without using any further treatment or surface passivating agents. The curry leaf seeds-based carbon dots (<i>Cls</i>-CDs) were synthesized further characterized by UV-visible, Photoluminescence (PL) spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), and X-ray photoelectron spectroscopy (XPS). The size of <i>Cls</i>-CDs was 3.89 ± 0.34 nm, and they exhibited blue-color emission at 455 nm upon excitation at 330 nm under UV illumination in solution with a quantum yield of 6.4%. In addition, <i>Cls</i>-CDs show excellent PL activity, excitation-dependent emission, and high pH stability. More interestingly, Fe<sup>3+</sup> is found to selectively quenching the PL of <i>Cls</i>-CDs over the several metal ions. The emission peak intensity of CDs at 455 nm was gradually quenched with increasing concentration of Fe<sup>3+</sup> ion, thereby Fe<sup>3+</sup> ion was quantified by plotting calibration curve over the range of 0–25 µM with a detection limit of 78.0 nM. The studies on reversibility confirmed that incorporating an inexpensive and easily obtainable EDTA ligand into the <i>Cls</i>-CDs leads to the restoration of PL, resulting in outstanding reusability for detecting Fe<sup>3+</sup> ions. Finally, the <i>Cls</i>-CDs are explored for cell imaging in HCT-116 cell lines using a MTT assay, revealing cell viabilities exceeding 90%, suggesting their great potential in bioimaging applications.</p>","PeriodicalId":146,"journal":{"name":"ChemistrySelect","volume":"10 23","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Curry Leaf Seeds (Murraya koenigii L.)-Derived Carbon Dots as Photoluminescent Probe for Nanomolar Detection of Fe3+ Ions and Bioimaging in HCT 116 Cancer Cells\",\"authors\":\"Diraviam Jeeva, Sonaimuthu Mohandoss, Kuppu Sakthi Velu, Naushad Ahmad, Mohammad Aslam, Govindaraju Bhuvanalogini, Seong-Cheol Kim\",\"doi\":\"10.1002/slct.202501909\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Carbon dots (CDs) synthesized from natural products have drawn numerous attention due to some unique properties. In this work, we synthesized photoluminescent carbon dots (CDs) using Curry leaf seeds (<i>Murraya koenigii</i> L.) via hydrothermal method at 140 °C for 6 h without using any further treatment or surface passivating agents. The curry leaf seeds-based carbon dots (<i>Cls</i>-CDs) were synthesized further characterized by UV-visible, Photoluminescence (PL) spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), and X-ray photoelectron spectroscopy (XPS). The size of <i>Cls</i>-CDs was 3.89 ± 0.34 nm, and they exhibited blue-color emission at 455 nm upon excitation at 330 nm under UV illumination in solution with a quantum yield of 6.4%. In addition, <i>Cls</i>-CDs show excellent PL activity, excitation-dependent emission, and high pH stability. More interestingly, Fe<sup>3+</sup> is found to selectively quenching the PL of <i>Cls</i>-CDs over the several metal ions. The emission peak intensity of CDs at 455 nm was gradually quenched with increasing concentration of Fe<sup>3+</sup> ion, thereby Fe<sup>3+</sup> ion was quantified by plotting calibration curve over the range of 0–25 µM with a detection limit of 78.0 nM. The studies on reversibility confirmed that incorporating an inexpensive and easily obtainable EDTA ligand into the <i>Cls</i>-CDs leads to the restoration of PL, resulting in outstanding reusability for detecting Fe<sup>3+</sup> ions. Finally, the <i>Cls</i>-CDs are explored for cell imaging in HCT-116 cell lines using a MTT assay, revealing cell viabilities exceeding 90%, suggesting their great potential in bioimaging applications.</p>\",\"PeriodicalId\":146,\"journal\":{\"name\":\"ChemistrySelect\",\"volume\":\"10 23\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-06-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ChemistrySelect\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/slct.202501909\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ChemistrySelect","FirstCategoryId":"92","ListUrlMain":"https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/slct.202501909","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Curry Leaf Seeds (Murraya koenigii L.)-Derived Carbon Dots as Photoluminescent Probe for Nanomolar Detection of Fe3+ Ions and Bioimaging in HCT 116 Cancer Cells
Carbon dots (CDs) synthesized from natural products have drawn numerous attention due to some unique properties. In this work, we synthesized photoluminescent carbon dots (CDs) using Curry leaf seeds (Murraya koenigii L.) via hydrothermal method at 140 °C for 6 h without using any further treatment or surface passivating agents. The curry leaf seeds-based carbon dots (Cls-CDs) were synthesized further characterized by UV-visible, Photoluminescence (PL) spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), and X-ray photoelectron spectroscopy (XPS). The size of Cls-CDs was 3.89 ± 0.34 nm, and they exhibited blue-color emission at 455 nm upon excitation at 330 nm under UV illumination in solution with a quantum yield of 6.4%. In addition, Cls-CDs show excellent PL activity, excitation-dependent emission, and high pH stability. More interestingly, Fe3+ is found to selectively quenching the PL of Cls-CDs over the several metal ions. The emission peak intensity of CDs at 455 nm was gradually quenched with increasing concentration of Fe3+ ion, thereby Fe3+ ion was quantified by plotting calibration curve over the range of 0–25 µM with a detection limit of 78.0 nM. The studies on reversibility confirmed that incorporating an inexpensive and easily obtainable EDTA ligand into the Cls-CDs leads to the restoration of PL, resulting in outstanding reusability for detecting Fe3+ ions. Finally, the Cls-CDs are explored for cell imaging in HCT-116 cell lines using a MTT assay, revealing cell viabilities exceeding 90%, suggesting their great potential in bioimaging applications.
期刊介绍:
ChemistrySelect is the latest journal from ChemPubSoc Europe and Wiley-VCH. It offers researchers a quality society-owned journal in which to publish their work in all areas of chemistry. Manuscripts are evaluated by active researchers to ensure they add meaningfully to the scientific literature, and those accepted are processed quickly to ensure rapid online publication.