54岁汉族胶质细胞瘤患者诱导多能干细胞TUSMi012-A的提取

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-06-08 DOI:10.1016/j.scr.2025.103753

Jiqiang Ma , Zhongwei Zhuang , Cheng Yang , Bei Zhang , Kuiming Zhang

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引用次数: 0

摘要

一位54岁男性胶质细胞瘤患者捐献外周血单个核细胞（pbmc）。使用仙台病毒与OKSM （OCT3/4、SOX2、KLF4和c-MYC）转录因子的非整合体对这些细胞进行重编程。经免疫细胞化学检测，获得的iPSCs具有多能性，核型保持正常，并能在体外分化为三种胚层。该iPSC系是研究胶质细胞瘤发病机制和开发治疗方法的重要工具。

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Derivation of induced pluripotent stem cell TUSMi012-A from a 54-year-old Chinese Han with gliocytoma

A 54-year-old male gliocytoma patient donated peripheral blood mononuclear cells (PBMCs). These cells were reprogrammed using a non-integrating Sendai Virus with OKSM (OCT3/4, SOX2, KLF4, and c-MYC) transcription factors. The resulting iPSCs showed pluripotency markers via immunocytochemistry, maintained a normal karyotype, and could differentiate into three germ layers in vitro. This iPSC line is a valuable tool for studying gliocytoma pathogenesis and developing treatments.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.