数字微流控平台中哺乳动物细胞培养与分析

IF 6.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Lab on a Chip Pub Date : 2025-06-13 DOI:10.1039/d5lc00250h

Burcu Gumuscu

{"title":"数字微流控平台中哺乳动物细胞培养与分析","authors":"Burcu Gumuscu","doi":"10.1039/d5lc00250h","DOIUrl":null,"url":null,"abstract":"Digital microfluidics has emerged as a promising platform for cell culture, offering precise control over droplet-based microenvironments while enabling automation and miniaturization. This review examines the integration of DMF with various cell culture substrates, including hydrogels and synthetic polymers, and evaluates their advantages and limitations for supporting cellular growth, adhesion, and viability. The influence of AC and DC actuation modes on droplet handling, as well as their effects on cell behavior, is also discussed. Furthermore, we highlight the incorporation of analytical tools and sorting techniques in DMF-based cell culture and explore its potential applications in organ-on-chip systems. While DMF presents unique opportunities for cell-based research, challenges such as material compatibility, cell viability, and system stability remain. This review provides a critical assessment of current developments and future directions for DMF in cell culture applications.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":"7 1","pages":""},"PeriodicalIF":6.1000,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mammalian cell culture and analysis in digital microfluidic platforms\",\"authors\":\"Burcu Gumuscu\",\"doi\":\"10.1039/d5lc00250h\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Digital microfluidics has emerged as a promising platform for cell culture, offering precise control over droplet-based microenvironments while enabling automation and miniaturization. This review examines the integration of DMF with various cell culture substrates, including hydrogels and synthetic polymers, and evaluates their advantages and limitations for supporting cellular growth, adhesion, and viability. The influence of AC and DC actuation modes on droplet handling, as well as their effects on cell behavior, is also discussed. Furthermore, we highlight the incorporation of analytical tools and sorting techniques in DMF-based cell culture and explore its potential applications in organ-on-chip systems. While DMF presents unique opportunities for cell-based research, challenges such as material compatibility, cell viability, and system stability remain. This review provides a critical assessment of current developments and future directions for DMF in cell culture applications.\",\"PeriodicalId\":85,\"journal\":{\"name\":\"Lab on a Chip\",\"volume\":\"7 1\",\"pages\":\"\"},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2025-06-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Lab on a Chip\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1039/d5lc00250h\",\"RegionNum\":2,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lab on a Chip","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1039/d5lc00250h","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

摘要

数字微流体已经成为细胞培养的一个有前途的平台，提供对基于液滴的微环境的精确控制，同时实现自动化和小型化。本文综述了DMF与各种细胞培养基质的结合，包括水凝胶和合成聚合物，并评估了它们在支持细胞生长、粘附和活力方面的优势和局限性。还讨论了交流和直流驱动模式对液滴处理的影响，以及它们对细胞行为的影响。此外，我们强调了dmf细胞培养中分析工具和分选技术的结合，并探索了其在器官芯片系统中的潜在应用。虽然DMF为基于细胞的研究提供了独特的机会，但材料相容性、细胞活力和系统稳定性等挑战仍然存在。本文综述了DMF在细胞培养中的应用现状和未来发展方向。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Mammalian cell culture and analysis in digital microfluidic platforms

Digital microfluidics has emerged as a promising platform for cell culture, offering precise control over droplet-based microenvironments while enabling automation and miniaturization. This review examines the integration of DMF with various cell culture substrates, including hydrogels and synthetic polymers, and evaluates their advantages and limitations for supporting cellular growth, adhesion, and viability. The influence of AC and DC actuation modes on droplet handling, as well as their effects on cell behavior, is also discussed. Furthermore, we highlight the incorporation of analytical tools and sorting techniques in DMF-based cell culture and explore its potential applications in organ-on-chip systems. While DMF presents unique opportunities for cell-based research, challenges such as material compatibility, cell viability, and system stability remain. This review provides a critical assessment of current developments and future directions for DMF in cell culture applications.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Lab on a Chip 工程技术-化学综合

CiteScore

11.10

自引率

8.20%

发文量

434

审稿时长

2.6 months

期刊介绍： Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.