哈密尔顿菌共生体通过调节母体蛋白tudor介导的piRNA通路,有利于粉虱受精。

IF 9.4 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Xiang Sun, Huan Li, Zhan-Bo Chen, Bing-Qi Liu, Chu-Qiao Li, Zheng-Yang Zhao, Xing-Ye Li, Jun-Bo Luan
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引用次数: 0

摘要

虽然人们普遍认为营养共生体可以操纵宿主繁殖,但其潜在的分子和细胞机制在很大程度上尚不清楚。细菌细胞中兼性共生体哈密尔顿菌诱导白蝇的雌偏性比。本研究表明,母系tudor基因(tud)及其编码蛋白在哈密通体白蝇卵巢中表达水平较低。Tud家族蛋白可以连接piwi相互作用RNA (piRNA)的生物合成的各个阶段,piRNA是一类小的非编码RNA。我们发现哈密通菌通过母体基因tud影响piRNA的丰度,从而调节空泡(H+)- atp酶H亚基(VATPH)的表达,这是空泡(H+)- atp酶活性的开关,在维持细胞内能量稳态和支持线粒体呼吸中起着至关重要的作用。这种调节调节了白蛉卵巢内ATP的水平。ATP水平决定了白蝇卵巢和卵中的f -肌动蛋白模式,最终控制了白蝇的受精。沉默剂通过损害卵巢和卵的ATP水平和f -肌动蛋白模式来抑制粉虱的受精。本研究揭示了共生体和母体蛋白结合可通过piRNA生物合成调节宿主受精。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hamiltonella symbionts benefit whitefly fertilization by regulating the maternal protein Tudor-mediated piRNA pathway.

Although it is widely recognized that nutritional symbionts can manipulate host reproduction, the underlying molecular and cellular mechanisms are largely unclear. The facultative symbiont Hamiltonella in bacteriocyte induces female-biased sex ratio of whiteflies. Here, we demonstrate that a maternal gene tudor (tud) and its encoded protein have lower expression levels in ovaries of Hamiltonella-cured whiteflies. Tud family proteins can interlink the various stages of biosynthesis of PIWI-interacting RNA (piRNA), a class of small noncoding RNAs. We find that Hamiltonella affects the abundance of a piRNA through the maternal gene tud, thereby regulating the expression of the vacuolar (H+)-ATPase H subunit (VATPH), which is the switch of activity of the vacuolar (H+)-ATPase that plays a crucial role in maintaining the homeostasis of intracellular energy and supporting mitochondrial respiration. This regulation adjusts the ATP level in ovaries of whiteflies. The ATP level shapes the F-actin pattern in ovaries and eggs of whiteflies, ultimately manipulating whitefly fertilization. Silencing tud inhibited whitefly fertilization by impairing ATP levels and F-actin patterns in ovaries and eggs. This study reveals that symbiont and maternal protein associations can regulate host fertilization by piRNA biosynthesis.

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来源期刊
CiteScore
19.00
自引率
0.90%
发文量
3575
审稿时长
2.5 months
期刊介绍: The Proceedings of the National Academy of Sciences (PNAS), a peer-reviewed journal of the National Academy of Sciences (NAS), serves as an authoritative source for high-impact, original research across the biological, physical, and social sciences. With a global scope, the journal welcomes submissions from researchers worldwide, making it an inclusive platform for advancing scientific knowledge.
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