Emily J Clarke, Anders Jensen, Alexandra M Gillen, David Bardell, Mark Senior, James R Anderson, Mandy J Peffers
{"title":"蛋白质组学分析揭示了异体马富血小板血浆和细胞外囊泡在调节肌腱炎症和修复中的潜在作用。","authors":"Emily J Clarke, Anders Jensen, Alexandra M Gillen, David Bardell, Mark Senior, James R Anderson, Mandy J Peffers","doi":"10.2460/ajvr.24.08.0241","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To determine the protein composition of equine platelet-rich plasma (PRP) and PRP-derived extracellular vesicles (EVs) and evaluate their effects on tendon inflammation in vitro. As tendon injuries are common in horses and treatment with PRP derived from the horse's own blood shows promise, but outcomes vary due to inconsistent composition. PRP contains EVs that facilitate cell communication.</p><p><strong>Methods: </strong>From December 2022 through May 2023, equine plasma (n = 3, adult) was isolated via double centrifugation and PRP produced using a commercial kit. Extracellular vesicles were isolated using differential ultracentrifugation and characterized with a tetraspanin assay. Equine tenocyte fibroblasts (n = 6) were stimulated with IL-1β and tumor necrosis factor-α for 24 hours to induce an inflammatory state simulating injury before treatment with PRP or PRP-derived EVs. Proteomic analysis employed data-dependent LC-MS-MS.</p><p><strong>Results: </strong>Compared to plasma, PRP and PRP EVs were enriched in proteins related to cellular waste disposal and inhibition of lipid metabolism. Experimental conditions significantly influenced the levels of 18 proteins in tenocyte fibroblasts. Collagen type 1 α chain 1 abundance decreased with treatment of PRP and PRP EVs, with implications for collagen metabolism. An increase in sequestosome 1 was also observed, having the potential to enhance inflammation or resolve it through autophagy-mediated degradation.</p><p><strong>Conclusions: </strong>PRP EVs influence the proteome of inflammatory tenocyte fibroblasts and may contribute to PRP's therapeutic effects.</p><p><strong>Clinical relevance: </strong>Understanding the protein composition of PRP and PRP-derived EVs may help optimize PRP-based treatments for tendon injuries. Therapies could become more consistent and effective, reducing reinjury rates and improving tendon healing.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-13"},"PeriodicalIF":1.3000,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Proteomic profiling reveals the potential role of allogenic equine platelet-rich plasma and extracellular vesicles in modulating tendon inflammation and repair.\",\"authors\":\"Emily J Clarke, Anders Jensen, Alexandra M Gillen, David Bardell, Mark Senior, James R Anderson, Mandy J Peffers\",\"doi\":\"10.2460/ajvr.24.08.0241\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To determine the protein composition of equine platelet-rich plasma (PRP) and PRP-derived extracellular vesicles (EVs) and evaluate their effects on tendon inflammation in vitro. As tendon injuries are common in horses and treatment with PRP derived from the horse's own blood shows promise, but outcomes vary due to inconsistent composition. PRP contains EVs that facilitate cell communication.</p><p><strong>Methods: </strong>From December 2022 through May 2023, equine plasma (n = 3, adult) was isolated via double centrifugation and PRP produced using a commercial kit. Extracellular vesicles were isolated using differential ultracentrifugation and characterized with a tetraspanin assay. Equine tenocyte fibroblasts (n = 6) were stimulated with IL-1β and tumor necrosis factor-α for 24 hours to induce an inflammatory state simulating injury before treatment with PRP or PRP-derived EVs. Proteomic analysis employed data-dependent LC-MS-MS.</p><p><strong>Results: </strong>Compared to plasma, PRP and PRP EVs were enriched in proteins related to cellular waste disposal and inhibition of lipid metabolism. Experimental conditions significantly influenced the levels of 18 proteins in tenocyte fibroblasts. Collagen type 1 α chain 1 abundance decreased with treatment of PRP and PRP EVs, with implications for collagen metabolism. An increase in sequestosome 1 was also observed, having the potential to enhance inflammation or resolve it through autophagy-mediated degradation.</p><p><strong>Conclusions: </strong>PRP EVs influence the proteome of inflammatory tenocyte fibroblasts and may contribute to PRP's therapeutic effects.</p><p><strong>Clinical relevance: </strong>Understanding the protein composition of PRP and PRP-derived EVs may help optimize PRP-based treatments for tendon injuries. Therapies could become more consistent and effective, reducing reinjury rates and improving tendon healing.</p>\",\"PeriodicalId\":7754,\"journal\":{\"name\":\"American journal of veterinary research\",\"volume\":\" \",\"pages\":\"1-13\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-06-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"American journal of veterinary research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2460/ajvr.24.08.0241\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2460/ajvr.24.08.0241","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Proteomic profiling reveals the potential role of allogenic equine platelet-rich plasma and extracellular vesicles in modulating tendon inflammation and repair.
Objective: To determine the protein composition of equine platelet-rich plasma (PRP) and PRP-derived extracellular vesicles (EVs) and evaluate their effects on tendon inflammation in vitro. As tendon injuries are common in horses and treatment with PRP derived from the horse's own blood shows promise, but outcomes vary due to inconsistent composition. PRP contains EVs that facilitate cell communication.
Methods: From December 2022 through May 2023, equine plasma (n = 3, adult) was isolated via double centrifugation and PRP produced using a commercial kit. Extracellular vesicles were isolated using differential ultracentrifugation and characterized with a tetraspanin assay. Equine tenocyte fibroblasts (n = 6) were stimulated with IL-1β and tumor necrosis factor-α for 24 hours to induce an inflammatory state simulating injury before treatment with PRP or PRP-derived EVs. Proteomic analysis employed data-dependent LC-MS-MS.
Results: Compared to plasma, PRP and PRP EVs were enriched in proteins related to cellular waste disposal and inhibition of lipid metabolism. Experimental conditions significantly influenced the levels of 18 proteins in tenocyte fibroblasts. Collagen type 1 α chain 1 abundance decreased with treatment of PRP and PRP EVs, with implications for collagen metabolism. An increase in sequestosome 1 was also observed, having the potential to enhance inflammation or resolve it through autophagy-mediated degradation.
Conclusions: PRP EVs influence the proteome of inflammatory tenocyte fibroblasts and may contribute to PRP's therapeutic effects.
Clinical relevance: Understanding the protein composition of PRP and PRP-derived EVs may help optimize PRP-based treatments for tendon injuries. Therapies could become more consistent and effective, reducing reinjury rates and improving tendon healing.
期刊介绍：
The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.
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