{"title":"一种基于二氧化锰纳米酶作为氧化酶模拟物的智能手机辅助比色纸传感器，用于快速评估食品中的总抗氧化能力。","authors":"Ruirui Shi, Chao Wang, Mingyue Zhu, Chen Xie, Wenyu Cui, Qing Zhou","doi":"10.1007/s00604-025-07277-9","DOIUrl":null,"url":null,"abstract":"<p><p>Manganese dioxide nanozymes (MnO<sub>2</sub>-zymes) with excellent oxidase-like properties were synthesized via a one-step solvothermal method. A rapid, portable smartphone-assisted colorimetric paper sensor based on MnO<sub>2</sub>-zymes/TMB chromogenic system was developed for total antioxidant capacity (TAC) evaluation. The MnO<sub>2</sub>-zymes immediately catalyzed the oxidation of colorless TMB to generate blue oxidation products (oxTMB), while antioxidants can quickly reduce oxTMB to TMB, causing noticeable fading of the blue color. Integrated with a smartphone-based RGB mode, ascorbic acid (AA) was quantified in the range 2.5-125.00 μM with a limit of detection (LOD) of 3.03 μM. Furthermore, using AA as a model analyte, the paper sensor was successfully applied to evaluate TAC in fruits and beverages, with results closely matching those obtained from a microplate reader-assisted colorimetric sensor and an ABTS free radical scavenging capacity assay kit. This high-performance sensor is simple, rapid, and accurate, showing great potential in practical application for TAC determination.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 7","pages":"414"},"PeriodicalIF":5.3000,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A smartphone-assisted colorimetric paper sensor based on MnO<sub>2</sub> nanozymes as oxidase mimetics for rapid evaluation of total antioxidant capacity in foods.\",\"authors\":\"Ruirui Shi, Chao Wang, Mingyue Zhu, Chen Xie, Wenyu Cui, Qing Zhou\",\"doi\":\"10.1007/s00604-025-07277-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Manganese dioxide nanozymes (MnO<sub>2</sub>-zymes) with excellent oxidase-like properties were synthesized via a one-step solvothermal method. A rapid, portable smartphone-assisted colorimetric paper sensor based on MnO<sub>2</sub>-zymes/TMB chromogenic system was developed for total antioxidant capacity (TAC) evaluation. The MnO<sub>2</sub>-zymes immediately catalyzed the oxidation of colorless TMB to generate blue oxidation products (oxTMB), while antioxidants can quickly reduce oxTMB to TMB, causing noticeable fading of the blue color. Integrated with a smartphone-based RGB mode, ascorbic acid (AA) was quantified in the range 2.5-125.00 μM with a limit of detection (LOD) of 3.03 μM. Furthermore, using AA as a model analyte, the paper sensor was successfully applied to evaluate TAC in fruits and beverages, with results closely matching those obtained from a microplate reader-assisted colorimetric sensor and an ABTS free radical scavenging capacity assay kit. This high-performance sensor is simple, rapid, and accurate, showing great potential in practical application for TAC determination.</p>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 7\",\"pages\":\"414\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-06-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1007/s00604-025-07277-9\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s00604-025-07277-9","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
A smartphone-assisted colorimetric paper sensor based on MnO2 nanozymes as oxidase mimetics for rapid evaluation of total antioxidant capacity in foods.
Manganese dioxide nanozymes (MnO2-zymes) with excellent oxidase-like properties were synthesized via a one-step solvothermal method. A rapid, portable smartphone-assisted colorimetric paper sensor based on MnO2-zymes/TMB chromogenic system was developed for total antioxidant capacity (TAC) evaluation. The MnO2-zymes immediately catalyzed the oxidation of colorless TMB to generate blue oxidation products (oxTMB), while antioxidants can quickly reduce oxTMB to TMB, causing noticeable fading of the blue color. Integrated with a smartphone-based RGB mode, ascorbic acid (AA) was quantified in the range 2.5-125.00 μM with a limit of detection (LOD) of 3.03 μM. Furthermore, using AA as a model analyte, the paper sensor was successfully applied to evaluate TAC in fruits and beverages, with results closely matching those obtained from a microplate reader-assisted colorimetric sensor and an ABTS free radical scavenging capacity assay kit. This high-performance sensor is simple, rapid, and accurate, showing great potential in practical application for TAC determination.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
