应用质粒id技术评价氮素固定效果及紫花苜蓿根系结瘤竞争力

IF 4 2区 生物学 Q2 MICROBIOLOGY
Jake D. Schumacher, Nicholas Dusek, Marcela Mendoza-Suárez, Barney A. Geddes
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引用次数: 0

摘要

最大限度地提高根瘤菌-豆科植物组合中的固氮作用,是可持续减少农业氮肥投入的一个机会。共生性状的高通量测量有可能加速鉴定根瘤菌/豆科植物的精英关联,并实现新的研究方法。质粒id技术最近应用于豆科根瘤菌,有助于同时评估根瘤菌的固氮效果和根瘤形成的竞争力。本研究将质粒id技术应用于根瘤菌中,这些根瘤菌是研究根瘤菌与豆科植物结合并与苜蓿形成重要经济共生关系的核心模型。开发了新的中国质粒id,并测试了其稳定性及其衡量根系结瘤和固氮效率竞争力的能力。根瘤菌的竞争力是通过使用下一代测序技术鉴定菌株特异性核苷酸条形码来测量的,而有效性是通过合成的nifH启动子驱动的GFP荧光来测量的。质粒id使研究人员能够同时有效地研究多种病原菌菌株的竞争力和有效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Adaptation of Plasmid-ID Technology for Evaluation of N2-Fixing Effectiveness and Competitiveness for Root Nodulation in the Sinorhizobium–Medicago System

Adaptation of Plasmid-ID Technology for Evaluation of N2-Fixing Effectiveness and Competitiveness for Root Nodulation in the Sinorhizobium–Medicago System

Adaptation of Plasmid-ID Technology for Evaluation of N2-Fixing Effectiveness and Competitiveness for Root Nodulation in the Sinorhizobium–Medicago System

Adaptation of Plasmid-ID Technology for Evaluation of N2-Fixing Effectiveness and Competitiveness for Root Nodulation in the Sinorhizobium–Medicago System

Adaptation of Plasmid-ID Technology for Evaluation of N2-Fixing Effectiveness and Competitiveness for Root Nodulation in the Sinorhizobium–Medicago System

Adaptation of Plasmid-ID Technology for Evaluation of N2-Fixing Effectiveness and Competitiveness for Root Nodulation in the Sinorhizobium–Medicago System

Maximising the nitrogen fixation occurring in rhizobia-legume associations represents an opportunity to sustainably reduce nitrogen fertiliser inputs in agriculture. High-throughput measurement of symbiotic traits has the potential to accelerate the identification of elite rhizobium/legume associations and enable novel research approaches. Plasmid-ID technology, recently deployed in Rhizobium leguminosarum, facilitates the concurrent assessment of rhizobium nitrogen-fixing effectiveness and competitiveness for root nodulation. This study adapts Plasmid-ID technology to function in Sinorhizobium species that are central models for studying rhizobium-legume associations and form economically important symbioses with alfalfa. New Sino-Plasmid-IDs were developed and tested for stability and their ability to measure competitiveness for root nodulation and nitrogen-fixing effectiveness. Rhizobial competitiveness is measured by identifying strain-specific nucleotide barcodes using next-generation sequencing, whereas effectiveness is measured by GFP fluorescence driven by the synthetic nifH promoter. Sino-Plasmid-IDs allow researchers to efficiently study competitiveness and effectiveness in a multitude of Sinorhizobium strains simultaneously.

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来源期刊
Environmental microbiology
Environmental microbiology 环境科学-微生物学
CiteScore
9.90
自引率
3.90%
发文量
427
审稿时长
2.3 months
期刊介绍: Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens
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