Yang Liu , Liuyan Yan , Lina Zhang , Xiaorui Tian , Shuai Zhang , Yingjie Guo , Yao Wang , Guoqing Fu , Zhenxing Zuo , Xiaojie Liu , Xiaoping Jia , Xuehai Zhu , Jincheng Yuan
{"title":"综合表型、细胞学、生理学和转录组学分析,为植物矮化提供了新的思路","authors":"Yang Liu , Liuyan Yan , Lina Zhang , Xiaorui Tian , Shuai Zhang , Yingjie Guo , Yao Wang , Guoqing Fu , Zhenxing Zuo , Xiaojie Liu , Xiaoping Jia , Xuehai Zhu , Jincheng Yuan","doi":"10.1016/j.plaphy.2025.110153","DOIUrl":null,"url":null,"abstract":"<div><div>Broomcorn millet is one of the most water-saving cereal crops. However, studies on regulatory mechanisms of its plant height that are closely associated with lodging resistance and yield are rarely reported. In this study, a dwarf mutant in broomcorn millet, designated as 778, was generated by ethyl methyl sulfonic acid (EMS) mutagenesis. Its dwarfing mechanism was analyzed using phenotypic, cytological and transcriptomic methods. 778 exhibited a dwarfing phenotype throughout its entire ontogenetic cycle. Compared with the wild type 260, 778 exhibited significant increases in tiller number and the antepenultimate leaf width, alongside a significant reduction in pedicel length, spike length and <span><span>1000-grain weight</span><svg><path></path></svg></span>. Cytological analysis of the main stem revealed decreases in the length of longitudinal cells. After spraying gibberellic acid 3 (GA<sub>3</sub>), the height of 778 significantly increased, confirming its classification as a GA<sub>3</sub>-sensitive dwarf mutant. Transcriptome analysis showed 337 differentially expressed genes (DEGs) between 778 and 260, which were mainly involved in four aspects according to enrichment of GO and KEGG. One dwarf-related gene <em>TD1</em> was cloned and sequenced, revealing that 778 lacked 58 bases before the termination codon. An antisense over-expression vector of <em>CLV1</em>, a <em>TD1</em> homologous gene, was constructed and transformed into <em>Arabidopsis thaliana</em>. It was observed that the height of transgenic plants was reduced, indicating that inhibition of <em>CLV1</em> expression might lead to dwarfing. Therefore, it is speculated that <em>TD1</em> mutation may be a critical factor contributing to the dwarfing of 778. This integrated analyses provides valuable insights into the dwarf mechanism of broomcorn millet.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110153"},"PeriodicalIF":6.1000,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Integrated phenotypic, cytological, physiological and transcriptomic analyses of Panicum miliaceum L. provide insights into the plant dwarfing\",\"authors\":\"Yang Liu , Liuyan Yan , Lina Zhang , Xiaorui Tian , Shuai Zhang , Yingjie Guo , Yao Wang , Guoqing Fu , Zhenxing Zuo , Xiaojie Liu , Xiaoping Jia , Xuehai Zhu , Jincheng Yuan\",\"doi\":\"10.1016/j.plaphy.2025.110153\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Broomcorn millet is one of the most water-saving cereal crops. However, studies on regulatory mechanisms of its plant height that are closely associated with lodging resistance and yield are rarely reported. In this study, a dwarf mutant in broomcorn millet, designated as 778, was generated by ethyl methyl sulfonic acid (EMS) mutagenesis. Its dwarfing mechanism was analyzed using phenotypic, cytological and transcriptomic methods. 778 exhibited a dwarfing phenotype throughout its entire ontogenetic cycle. Compared with the wild type 260, 778 exhibited significant increases in tiller number and the antepenultimate leaf width, alongside a significant reduction in pedicel length, spike length and <span><span>1000-grain weight</span><svg><path></path></svg></span>. Cytological analysis of the main stem revealed decreases in the length of longitudinal cells. After spraying gibberellic acid 3 (GA<sub>3</sub>), the height of 778 significantly increased, confirming its classification as a GA<sub>3</sub>-sensitive dwarf mutant. Transcriptome analysis showed 337 differentially expressed genes (DEGs) between 778 and 260, which were mainly involved in four aspects according to enrichment of GO and KEGG. One dwarf-related gene <em>TD1</em> was cloned and sequenced, revealing that 778 lacked 58 bases before the termination codon. An antisense over-expression vector of <em>CLV1</em>, a <em>TD1</em> homologous gene, was constructed and transformed into <em>Arabidopsis thaliana</em>. It was observed that the height of transgenic plants was reduced, indicating that inhibition of <em>CLV1</em> expression might lead to dwarfing. Therefore, it is speculated that <em>TD1</em> mutation may be a critical factor contributing to the dwarfing of 778. 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Integrated phenotypic, cytological, physiological and transcriptomic analyses of Panicum miliaceum L. provide insights into the plant dwarfing
Broomcorn millet is one of the most water-saving cereal crops. However, studies on regulatory mechanisms of its plant height that are closely associated with lodging resistance and yield are rarely reported. In this study, a dwarf mutant in broomcorn millet, designated as 778, was generated by ethyl methyl sulfonic acid (EMS) mutagenesis. Its dwarfing mechanism was analyzed using phenotypic, cytological and transcriptomic methods. 778 exhibited a dwarfing phenotype throughout its entire ontogenetic cycle. Compared with the wild type 260, 778 exhibited significant increases in tiller number and the antepenultimate leaf width, alongside a significant reduction in pedicel length, spike length and 1000-grain weight. Cytological analysis of the main stem revealed decreases in the length of longitudinal cells. After spraying gibberellic acid 3 (GA3), the height of 778 significantly increased, confirming its classification as a GA3-sensitive dwarf mutant. Transcriptome analysis showed 337 differentially expressed genes (DEGs) between 778 and 260, which were mainly involved in four aspects according to enrichment of GO and KEGG. One dwarf-related gene TD1 was cloned and sequenced, revealing that 778 lacked 58 bases before the termination codon. An antisense over-expression vector of CLV1, a TD1 homologous gene, was constructed and transformed into Arabidopsis thaliana. It was observed that the height of transgenic plants was reduced, indicating that inhibition of CLV1 expression might lead to dwarfing. Therefore, it is speculated that TD1 mutation may be a critical factor contributing to the dwarfing of 778. This integrated analyses provides valuable insights into the dwarf mechanism of broomcorn millet.
期刊介绍:
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.