{"title":"miR-8调节中国犰狳的免疫防御和生长:对病原体抗性和生物防治优化的影响","authors":"Ximei Yuan, Yiqing He, Chen Wang, Yuejun Fu","doi":"10.1016/j.jip.2025.108384","DOIUrl":null,"url":null,"abstract":"<div><div><em>Arma chinensis</em> Fallou is a critical biocontrol agent for agricultural pests; nevertheless, its effectiveness is limited by susceptibility to pathogens. The development of artificial diets is anticipated to address this challenge, necessitating a systematic investigation into the physiological and molecular regulatory mechanisms underlying immunity and developmental processes in <em>A. chinensis</em>. This approach further requires the identification of genes and regulatory molecules critical for maintaining immune homeostasis and developmental progression in this species. Using RNAi technology, paraffin sectioning, HE staining, and metabolomic sequencing, the regulatory mechanisms of miR-8 on the immunity and growth of the natural enemy insect <em>A. chinensis</em> were revealed at multiple levels, including individual, cellular, molecular, and metabolic levels. <em>A. chinensis</em> resistance to viral infection by activating innate immunity pathways while enhancing antimicrobial peptide <em>Ach</em>Defensin production. Meanwhile, host miR-8 can inhibit the proliferation of AcMNPV. Metabolomic profiling revealed miR-8′s systemic influence on bile acid biosynthesis, actin cytoskeleton dynamics, and insulin secretion, which coordinate nutrient utilisation and growth <em>via</em> mTOR and IIS signalling. Overexpression of miR-8 significantly increased host body size, shortened developmental time, and promoted fat body expansion. This study reveals miR-8 as a pivotal regulator of <em>A. chinensis</em> immunity and development, presenting innovative options to improve the cost-effectiveness of biological control. These findings enhance the comprehension of miRNA-mediated pathogen resistance in beneficial insects, with ramifications for sustainable pest management and invertebrate disease control.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"212 ","pages":"Article 108384"},"PeriodicalIF":2.4000,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"miR-8 modulates immune defense and growth in Arma chinensis Fallou: Implications for pathogen resistance and biocontrol optimization\",\"authors\":\"Ximei Yuan, Yiqing He, Chen Wang, Yuejun Fu\",\"doi\":\"10.1016/j.jip.2025.108384\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Arma chinensis</em> Fallou is a critical biocontrol agent for agricultural pests; nevertheless, its effectiveness is limited by susceptibility to pathogens. The development of artificial diets is anticipated to address this challenge, necessitating a systematic investigation into the physiological and molecular regulatory mechanisms underlying immunity and developmental processes in <em>A. chinensis</em>. This approach further requires the identification of genes and regulatory molecules critical for maintaining immune homeostasis and developmental progression in this species. Using RNAi technology, paraffin sectioning, HE staining, and metabolomic sequencing, the regulatory mechanisms of miR-8 on the immunity and growth of the natural enemy insect <em>A. chinensis</em> were revealed at multiple levels, including individual, cellular, molecular, and metabolic levels. <em>A. chinensis</em> resistance to viral infection by activating innate immunity pathways while enhancing antimicrobial peptide <em>Ach</em>Defensin production. Meanwhile, host miR-8 can inhibit the proliferation of AcMNPV. Metabolomic profiling revealed miR-8′s systemic influence on bile acid biosynthesis, actin cytoskeleton dynamics, and insulin secretion, which coordinate nutrient utilisation and growth <em>via</em> mTOR and IIS signalling. Overexpression of miR-8 significantly increased host body size, shortened developmental time, and promoted fat body expansion. This study reveals miR-8 as a pivotal regulator of <em>A. chinensis</em> immunity and development, presenting innovative options to improve the cost-effectiveness of biological control. These findings enhance the comprehension of miRNA-mediated pathogen resistance in beneficial insects, with ramifications for sustainable pest management and invertebrate disease control.</div></div>\",\"PeriodicalId\":16296,\"journal\":{\"name\":\"Journal of invertebrate pathology\",\"volume\":\"212 \",\"pages\":\"Article 108384\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-06-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of invertebrate pathology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0022201125001181\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of invertebrate pathology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022201125001181","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ZOOLOGY","Score":null,"Total":0}
miR-8 modulates immune defense and growth in Arma chinensis Fallou: Implications for pathogen resistance and biocontrol optimization
Arma chinensis Fallou is a critical biocontrol agent for agricultural pests; nevertheless, its effectiveness is limited by susceptibility to pathogens. The development of artificial diets is anticipated to address this challenge, necessitating a systematic investigation into the physiological and molecular regulatory mechanisms underlying immunity and developmental processes in A. chinensis. This approach further requires the identification of genes and regulatory molecules critical for maintaining immune homeostasis and developmental progression in this species. Using RNAi technology, paraffin sectioning, HE staining, and metabolomic sequencing, the regulatory mechanisms of miR-8 on the immunity and growth of the natural enemy insect A. chinensis were revealed at multiple levels, including individual, cellular, molecular, and metabolic levels. A. chinensis resistance to viral infection by activating innate immunity pathways while enhancing antimicrobial peptide AchDefensin production. Meanwhile, host miR-8 can inhibit the proliferation of AcMNPV. Metabolomic profiling revealed miR-8′s systemic influence on bile acid biosynthesis, actin cytoskeleton dynamics, and insulin secretion, which coordinate nutrient utilisation and growth via mTOR and IIS signalling. Overexpression of miR-8 significantly increased host body size, shortened developmental time, and promoted fat body expansion. This study reveals miR-8 as a pivotal regulator of A. chinensis immunity and development, presenting innovative options to improve the cost-effectiveness of biological control. These findings enhance the comprehension of miRNA-mediated pathogen resistance in beneficial insects, with ramifications for sustainable pest management and invertebrate disease control.
期刊介绍:
The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates.
The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.