脂质体纳米捕集剂中和单核增生李斯特菌毒素增强巨噬细胞活力和抗菌能力。

IF 1.8 Q3 INFECTIOUS DISEASES
Hervé Besançon, Margherita Polidori, Andrea Hostettler, Victor Nizet, Anna Oevermann, Eduard Babiychuk
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引用次数: 0

摘要

单核细胞增生李斯特菌是一种人类和动物病原体,是世界范围内食源性感染最常见的病原体之一。它会导致严重的并发症,如脑膜炎或流产。抗毒力疗法,其目标毒力因素,如成孔毒素,提供了另一种方法来对抗感染。在这项研究中,含胆固醇的脂质体纳米陷阱有效地中和了单核增生乳杆菌的外毒素,特别是李斯特菌溶素O (LLO),从而保护了哺乳动物细胞。值得注意的是,在中性和酸性条件下都观察到毒素中和,其中LLO活性被优化以促进细菌从吞噬体中逃逸。脂质体纳米陷阱被巨噬细胞吞噬并与细胞内李斯特菌共定位,提高了细胞内细菌的清除率。这些发现扩大了广谱脂质体纳米陷阱疗法的潜在应用,它可以与目前的标准护理治疗一起使用,以帮助免疫系统控制毒性病原体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Liposomal Nanotraps Neutralize Listeria monocytogenes Toxins to Enhance Macrophage Viability and Antibacterial Capacity.

Listeria monocytogenes is a human and veterinary pathogen, one of the most common agents of foodborne infections worldwide. It can cause severe complications such as meningitis or miscarriage. Anti-virulence therapies, which target virulence factors such as pore-forming toxins, offer an alternative approach to combating infections. In this study, cholesterol-containing liposomal nanotraps effectively neutralized L. monocytogenes exotoxins, particularly listeriolysin O (LLO), thereby protecting mammalian cells. Notably, toxin neutralization was observed under both neutral and acidic conditions, where LLO activity is optimized to facilitate bacterial escape from the phagosome. Liposomal nanotraps were phagocytosed by macrophages and colocalized with intracellular Listeria, increasing the clearance rate of intracellular bacteria. These findings expand the potential use of broad-spectrum liposomal nanotrap therapy, which could be employed alongside current standard of care treatments to assist the immune system in controlling virulent pathogens.

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