通过Oligo-LiveFISH可视化活细胞中的染色质通讯。

IF 4.5 Q1 BIOCHEMICAL RESEARCH METHODS

Cell Reports Methods Pub Date : 2025-06-16 Epub Date: 2025-06-09 DOI:10.1016/j.crmeth.2025.101079

Mattia Conte, Mario Nicodemi

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引用次数: 0

摘要

了解基因组调控需要实时捕获染色质动力学。在最近一期的Cell杂志上，Zhu等人介绍了Oligo-LiveFISH，1这是一种高分辨率的活细胞成像平台，无需基因组工程即可跟踪非重复基因位点。通过将荧光引导RNA池与dCas9组装在一起，该方法可以实现20 nm， 50 ms分辨率的成像，从而深入了解细胞系和原代细胞之间的动态基因组组织。

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Visualizing chromatin communication in living cells through Oligo-LiveFISH.

Understanding genome regulation requires capturing chromatin dynamics in real time. In a recent issue of Cell, Zhu et al. introduce Oligo-LiveFISH,¹ a high-resolution live-cell imaging platform that tracks non-repetitive loci without genome engineering. By assembling fluorescent guide RNA pools with dCas9, this method enables 20-nm, 50-ms resolution imaging, offering insights into dynamic genome organization across cell lines and primary cells.

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来源期刊

Cell Reports Methods Chemistry (General), Biochemistry, Genetics and Molecular Biology (General), Immunology and Microbiology (General)

CiteScore

3.80

自引率

0.00%

发文量

审稿时长

111 days