{"title":"玉米花药中21-nt相rna对靶mRNA裂解的时空调控。","authors":"Bi Lian, Tianxiao Huang, Wei Huang, Pengfei Jiang, Weiwei Jin, Yijun Qi","doi":"10.1073/pnas.2422647122","DOIUrl":null,"url":null,"abstract":"<p><p>21-nt phasiRNAs are abundantly expressed in anthers and play crucial roles in anther development and male reproduction in grasses. In maize, the target genes that 21-nt phasiRNAs regulate remain largely unknown. Here, we show that 21-nt phasiRNAs direct the cleavage of hundreds of target mRNAs in maize. Interestingly, while 21-nt phasiRNAs in anthers (somatic anther wall cells) do not have obvious preference for a particular nucleotide at the 5' end and target genes implicated in stress response and cellular homeostasis, 21-nt phasiRNAs in male germ cells preferentially initiate with 5' C and target genes related to meiotic processes in a ZmAGO5c-dependent manner. Our results indicate that mRNA cleavage is a conserved mechanism used by 21-nt phasiRNAs for gene regulation in grasses. However, the sequences of 21-nt phasiRNAs and their targets show little conservation in maize and rice, suggesting that <i>21PHAS</i> loci and 21-nt phasiRNA targets have undergone fast divergence in grasses.</p>","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"122 24","pages":"e2422647122"},"PeriodicalIF":9.4000,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Spatiotemporal regulation of target mRNA cleavage by 21-nt phasiRNAs in maize anthers.\",\"authors\":\"Bi Lian, Tianxiao Huang, Wei Huang, Pengfei Jiang, Weiwei Jin, Yijun Qi\",\"doi\":\"10.1073/pnas.2422647122\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>21-nt phasiRNAs are abundantly expressed in anthers and play crucial roles in anther development and male reproduction in grasses. In maize, the target genes that 21-nt phasiRNAs regulate remain largely unknown. Here, we show that 21-nt phasiRNAs direct the cleavage of hundreds of target mRNAs in maize. Interestingly, while 21-nt phasiRNAs in anthers (somatic anther wall cells) do not have obvious preference for a particular nucleotide at the 5' end and target genes implicated in stress response and cellular homeostasis, 21-nt phasiRNAs in male germ cells preferentially initiate with 5' C and target genes related to meiotic processes in a ZmAGO5c-dependent manner. Our results indicate that mRNA cleavage is a conserved mechanism used by 21-nt phasiRNAs for gene regulation in grasses. However, the sequences of 21-nt phasiRNAs and their targets show little conservation in maize and rice, suggesting that <i>21PHAS</i> loci and 21-nt phasiRNA targets have undergone fast divergence in grasses.</p>\",\"PeriodicalId\":20548,\"journal\":{\"name\":\"Proceedings of the National Academy of Sciences of the United States of America\",\"volume\":\"122 24\",\"pages\":\"e2422647122\"},\"PeriodicalIF\":9.4000,\"publicationDate\":\"2025-06-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of the National Academy of Sciences of the United States of America\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://doi.org/10.1073/pnas.2422647122\",\"RegionNum\":1,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/6/11 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the National Academy of Sciences of the United States of America","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.1073/pnas.2422647122","RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/11 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
Spatiotemporal regulation of target mRNA cleavage by 21-nt phasiRNAs in maize anthers.
21-nt phasiRNAs are abundantly expressed in anthers and play crucial roles in anther development and male reproduction in grasses. In maize, the target genes that 21-nt phasiRNAs regulate remain largely unknown. Here, we show that 21-nt phasiRNAs direct the cleavage of hundreds of target mRNAs in maize. Interestingly, while 21-nt phasiRNAs in anthers (somatic anther wall cells) do not have obvious preference for a particular nucleotide at the 5' end and target genes implicated in stress response and cellular homeostasis, 21-nt phasiRNAs in male germ cells preferentially initiate with 5' C and target genes related to meiotic processes in a ZmAGO5c-dependent manner. Our results indicate that mRNA cleavage is a conserved mechanism used by 21-nt phasiRNAs for gene regulation in grasses. However, the sequences of 21-nt phasiRNAs and their targets show little conservation in maize and rice, suggesting that 21PHAS loci and 21-nt phasiRNA targets have undergone fast divergence in grasses.
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The Proceedings of the National Academy of Sciences (PNAS), a peer-reviewed journal of the National Academy of Sciences (NAS), serves as an authoritative source for high-impact, original research across the biological, physical, and social sciences. With a global scope, the journal welcomes submissions from researchers worldwide, making it an inclusive platform for advancing scientific knowledge.
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