{"title":"利用等离子体增强纳米生物传感技术实时监测诱导多能干细胞神经元分化过程中的miRNA动态。","authors":"Yannan Hou, Meizi Chen, Letao Yang, Ki-Bum Lee","doi":"10.1021/acs.nanolett.5c01840","DOIUrl":null,"url":null,"abstract":"<p><p>Induced pluripotent stem cells (iPSCs) offer immense potential for treating central nervous system (CNS) disorders and injuries. However, the lack of highly sensitive, selective, and noninvasive biosensors for real-time monitoring of iPSC neuronal differentiation remains a critical barrier. In this work, we introduce a gold nanorod-based metal-enhanced molecular beacon (MEMB) nanobiosensor for the noninvasive, real-time detection of intracellular miRNA-124, a key biomarker for neuronal differentiation in human iPSC-derived neural stem cells. Designed through finite-difference time-domain (FDTD) simulations and experimentally validated for optimized localized surface plasmon resonance (LSPR) properties, MEMB nanobiosensors achieved picomolar-level sensitivity and single-mismatch selectivity toward miRNA-124 detection, along with great biocompatibility demonstrated by live-cell assays. Collectively, the MEMB platform provides a robust analytical tool for in-depth investigations of molecular and genetic regulatory networks during iPSC neuronal differentiation in a nondestructive manner, paving the way toward safer, more efficient, and better-characterized iPSC-derived cell therapies for CNS diseases and injuries.</p>","PeriodicalId":53,"journal":{"name":"Nano Letters","volume":" ","pages":"10402-10411"},"PeriodicalIF":9.6000,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Real-Time Intracellular Monitoring of miRNA Dynamics during Induced Pluripotent Stem Cell Neuronal Differentiation via Plasmon-Enhanced Nanobiosensing.\",\"authors\":\"Yannan Hou, Meizi Chen, Letao Yang, Ki-Bum Lee\",\"doi\":\"10.1021/acs.nanolett.5c01840\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Induced pluripotent stem cells (iPSCs) offer immense potential for treating central nervous system (CNS) disorders and injuries. However, the lack of highly sensitive, selective, and noninvasive biosensors for real-time monitoring of iPSC neuronal differentiation remains a critical barrier. In this work, we introduce a gold nanorod-based metal-enhanced molecular beacon (MEMB) nanobiosensor for the noninvasive, real-time detection of intracellular miRNA-124, a key biomarker for neuronal differentiation in human iPSC-derived neural stem cells. Designed through finite-difference time-domain (FDTD) simulations and experimentally validated for optimized localized surface plasmon resonance (LSPR) properties, MEMB nanobiosensors achieved picomolar-level sensitivity and single-mismatch selectivity toward miRNA-124 detection, along with great biocompatibility demonstrated by live-cell assays. Collectively, the MEMB platform provides a robust analytical tool for in-depth investigations of molecular and genetic regulatory networks during iPSC neuronal differentiation in a nondestructive manner, paving the way toward safer, more efficient, and better-characterized iPSC-derived cell therapies for CNS diseases and injuries.</p>\",\"PeriodicalId\":53,\"journal\":{\"name\":\"Nano Letters\",\"volume\":\" \",\"pages\":\"10402-10411\"},\"PeriodicalIF\":9.6000,\"publicationDate\":\"2025-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nano Letters\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://doi.org/10.1021/acs.nanolett.5c01840\",\"RegionNum\":1,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/6/10 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nano Letters","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1021/acs.nanolett.5c01840","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/10 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Real-Time Intracellular Monitoring of miRNA Dynamics during Induced Pluripotent Stem Cell Neuronal Differentiation via Plasmon-Enhanced Nanobiosensing.
Induced pluripotent stem cells (iPSCs) offer immense potential for treating central nervous system (CNS) disorders and injuries. However, the lack of highly sensitive, selective, and noninvasive biosensors for real-time monitoring of iPSC neuronal differentiation remains a critical barrier. In this work, we introduce a gold nanorod-based metal-enhanced molecular beacon (MEMB) nanobiosensor for the noninvasive, real-time detection of intracellular miRNA-124, a key biomarker for neuronal differentiation in human iPSC-derived neural stem cells. Designed through finite-difference time-domain (FDTD) simulations and experimentally validated for optimized localized surface plasmon resonance (LSPR) properties, MEMB nanobiosensors achieved picomolar-level sensitivity and single-mismatch selectivity toward miRNA-124 detection, along with great biocompatibility demonstrated by live-cell assays. Collectively, the MEMB platform provides a robust analytical tool for in-depth investigations of molecular and genetic regulatory networks during iPSC neuronal differentiation in a nondestructive manner, paving the way toward safer, more efficient, and better-characterized iPSC-derived cell therapies for CNS diseases and injuries.
期刊介绍：
Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including:
- Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale
- Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies
- Modeling and simulation of synthetic, assembly, and interaction processes
- Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance
- Applications of nanoscale materials in living and environmental systems
Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.
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