香豆属植物香豆素的分离纯化稳定。以及它们的抗神经炎活性

IF 3.4 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Phytochemistry Pub Date : 2025-06-10 DOI:10.1016/j.phytochem.2025.114579

Huadong Li , Wenxin Zhang , Junjie Cai , Luxi Chen , Yihai Wang , Jingwen Xu , Xiangjiu He

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引用次数: 0

摘要

仙人掌的空中部分。稳定。经水萃取、系统分离和用序贯色谱技术纯化。获得了21种香豆素，包括6种以前未描述过的香豆素。基于HR-ESI-MS、NMR和ECD数据的综合分析，对它们的结构进行了鉴定。采用脂多糖诱导的BV-2小胶质细胞神经炎症模型，对分离的香豆素的抗神经炎症活性进行了评估。Fraxin甲醚(2)对一氧化氮（NO）产生有明显的抑制作用，IC50值为17.07±2.88 μM。机制研究表明，2抑制NF-κB核易位，下调促炎介质（iNOS、COX-2）和细胞因子（IL-1β、IL-6）的表达，从而减轻神经炎症反应。这些发现为进一步开发和利用草属植物作为潜在抗神经炎药物的天然来源提供了植物化学基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Coumarins isolated from Paederia scandens (Lour.) Merr. and their anti-neuroinflammatory activity

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Coumarins isolated from Paederia scandens (Lour.) Merr. and their anti-neuroinflammatory activity

The aerial parts of Paederia scandens (Lour.) Merr. were subjected to aqueous extraction followed by systematic isolation and purification using sequential chromatographic techniques. Twenty-one coumarins, including six previously undescribed ones, were obtained. Their structures were elucidated based on comprehensive analyses of HR-ESI-MS, NMR and ECD data. The anti-neuroinflammatory activity of the isolated coumarins was evaluated using an LPS-induced neuroinflammation model in BV-2 microglia cells. Fraxin methyl ether (2) demonstrated significant inhibition of nitric oxide (NO) production, with an IC₅₀ value of 17.07 ± 2.88 μM. Mechanistic investigations revealed that 2 suppressed the nuclear translocation of NF-κB and downregulated the expression of pro-inflammatory mediators (iNOS, COX-2) and cytokines (IL-1β, IL-6), thereby attenuating neuroinflammatory responses. These findings provide a phytochemical basis for further development and utilization of P. scandens as a natural source of potential anti-neuroinflammatory agents.

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来源期刊

Phytochemistry 生物-植物科学

CiteScore

6.40

自引率

7.90%

发文量

443

审稿时长

39 days

期刊介绍： Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.